中国组织工程研究与临床康复
中國組織工程研究與臨床康複
중국조직공정연구여림상강복
JOURNAL OF CLINICAL REHABILITATIVE TISSUE ENGINEERING RESEARCH
2007年
29期
5857-5860
,共4页
背景:糖尿病性肢端坏疽已成为糖尿病致残、致死的重要原因之一.目的:建立糖尿病性肢端坏疽的大鼠模型,观察中药复方对糖尿病性肢端坏疽的干预作用.设计:对比观察实验.单位:河南科技大学临床医学院第一附属医院.材料:选用雄性Wistar大鼠50只,6周龄,体质量(200.0±16.3)g.以普通饲料单笼喂养,室温18~22℃,自由摄食、饮水.随机抽取10只作为空白对照组,其余40只用于造模.方法:实验于2001-10/2002-04在河南科技大学动物房完成.①实验分组:将40只大鼠禁食6 h后左下腹腹腔注射链脲佐菌素55.0 mg/kg;空白对照组注入同体积的柠檬酸钠缓冲溶液.选用造模成功的20只大鼠,随机分为2组:模型组和治疗组,每组10只.治疗组大鼠于造模成功后每天9:00灌胃中药(60 g/kg),共3周;模型组灌胃等体积的生理盐水.第3周末,各组大鼠禁食12 h,麻醉后处死取血测血糖、血脂和胰岛素水平;实验过程中记录各组大鼠的每日饮水量.②实验评估:肢端坏疽积分标准:以皮肤颜色发黑、皮肤有轻度开放性病灶、病灶已侵入深部肌肉组织3个等级对糖尿病性肢端坏疽大鼠的四肢进行评分,计算每只大鼠的总积分.对胰岛β细胞分泌的功能进行评价主要观察指标:实验前后大鼠饮水量、体质量、三酰甘油、胆固醇、空腹血糖、血脂和胰岛素水平的变化.结果:纳入Wistar大鼠50只,造模不成功脱落20只,30只进入结果分析.①实验期间,模型组和治疗组饮水量明显高于空白对照组(P<0.01);治疗组随着治疗时间增加饮水量明显下降,模型组随着时间的推移逐渐升高.②治疗后模型组大鼠体质量明显低于治疗前(P<0.01),治疗组有下降趋势,与治疗前比较,差异不明显(P>0.05).③两组大鼠均有明显肢端坏疽出现(P<0.01).治疗组大鼠的体质量明显高于模型组(P<0.01),肢端坏疽情况明显好于模型组(P<0.01).④治疗前治疗组和模型组的空腹血糖水平明显高于空白对照组(P<0.01),而胰岛素水平和胰岛β细胞功能指数明显低于空白对照组(P<0.01).治疗后两组的空腹血糖水平高于空白对照组(P<0.01),治疗组的明显低于模型组(P<0.01),已接近正常值;两组血脂和胰岛β细胞功能指数均明显低于空白对照组(P<0.01),治疗组明显高于模型组(P<0.01),接近正常值.⑤治疗前其它两组三酰甘油、胆固醇水平明显高于空白对照组(P<0.01).经过治疗,治疗组三酰甘油、胆固醇明显下降(P<0.01),与空白对照组比较,差异不明显(P>0.05);模型组继续升高,明显高于空白对照组(P<0.01).结论:血清胰岛素水平升高,血糖、血脂水平下降,可在一定程度上防治糖尿病足的发生、发展.该糖尿病性肢端坏疽模型对药物反应敏感,可用于研究糖尿病足发病机制及药物治疗效果的评价.
揹景:糖尿病性肢耑壞疽已成為糖尿病緻殘、緻死的重要原因之一.目的:建立糖尿病性肢耑壞疽的大鼠模型,觀察中藥複方對糖尿病性肢耑壞疽的榦預作用.設計:對比觀察實驗.單位:河南科技大學臨床醫學院第一附屬醫院.材料:選用雄性Wistar大鼠50隻,6週齡,體質量(200.0±16.3)g.以普通飼料單籠餵養,室溫18~22℃,自由攝食、飲水.隨機抽取10隻作為空白對照組,其餘40隻用于造模.方法:實驗于2001-10/2002-04在河南科技大學動物房完成.①實驗分組:將40隻大鼠禁食6 h後左下腹腹腔註射鏈脲佐菌素55.0 mg/kg;空白對照組註入同體積的檸檬痠鈉緩遲溶液.選用造模成功的20隻大鼠,隨機分為2組:模型組和治療組,每組10隻.治療組大鼠于造模成功後每天9:00灌胃中藥(60 g/kg),共3週;模型組灌胃等體積的生理鹽水.第3週末,各組大鼠禁食12 h,痳醉後處死取血測血糖、血脂和胰島素水平;實驗過程中記錄各組大鼠的每日飲水量.②實驗評估:肢耑壞疽積分標準:以皮膚顏色髮黑、皮膚有輕度開放性病竈、病竈已侵入深部肌肉組織3箇等級對糖尿病性肢耑壞疽大鼠的四肢進行評分,計算每隻大鼠的總積分.對胰島β細胞分泌的功能進行評價主要觀察指標:實驗前後大鼠飲水量、體質量、三酰甘油、膽固醇、空腹血糖、血脂和胰島素水平的變化.結果:納入Wistar大鼠50隻,造模不成功脫落20隻,30隻進入結果分析.①實驗期間,模型組和治療組飲水量明顯高于空白對照組(P<0.01);治療組隨著治療時間增加飲水量明顯下降,模型組隨著時間的推移逐漸升高.②治療後模型組大鼠體質量明顯低于治療前(P<0.01),治療組有下降趨勢,與治療前比較,差異不明顯(P>0.05).③兩組大鼠均有明顯肢耑壞疽齣現(P<0.01).治療組大鼠的體質量明顯高于模型組(P<0.01),肢耑壞疽情況明顯好于模型組(P<0.01).④治療前治療組和模型組的空腹血糖水平明顯高于空白對照組(P<0.01),而胰島素水平和胰島β細胞功能指數明顯低于空白對照組(P<0.01).治療後兩組的空腹血糖水平高于空白對照組(P<0.01),治療組的明顯低于模型組(P<0.01),已接近正常值;兩組血脂和胰島β細胞功能指數均明顯低于空白對照組(P<0.01),治療組明顯高于模型組(P<0.01),接近正常值.⑤治療前其它兩組三酰甘油、膽固醇水平明顯高于空白對照組(P<0.01).經過治療,治療組三酰甘油、膽固醇明顯下降(P<0.01),與空白對照組比較,差異不明顯(P>0.05);模型組繼續升高,明顯高于空白對照組(P<0.01).結論:血清胰島素水平升高,血糖、血脂水平下降,可在一定程度上防治糖尿病足的髮生、髮展.該糖尿病性肢耑壞疽模型對藥物反應敏感,可用于研究糖尿病足髮病機製及藥物治療效果的評價.
배경:당뇨병성지단배저이성위당뇨병치잔、치사적중요원인지일.목적:건립당뇨병성지단배저적대서모형,관찰중약복방대당뇨병성지단배저적간예작용.설계:대비관찰실험.단위:하남과기대학림상의학원제일부속의원.재료:선용웅성Wistar대서50지,6주령,체질량(200.0±16.3)g.이보통사료단롱위양,실온18~22℃,자유섭식、음수.수궤추취10지작위공백대조조,기여40지용우조모.방법:실험우2001-10/2002-04재하남과기대학동물방완성.①실험분조:장40지대서금식6 h후좌하복복강주사련뇨좌균소55.0 mg/kg;공백대조조주입동체적적저몽산납완충용액.선용조모성공적20지대서,수궤분위2조:모형조화치료조,매조10지.치료조대서우조모성공후매천9:00관위중약(60 g/kg),공3주;모형조관위등체적적생리염수.제3주말,각조대서금식12 h,마취후처사취혈측혈당、혈지화이도소수평;실험과정중기록각조대서적매일음수량.②실험평고:지단배저적분표준:이피부안색발흑、피부유경도개방성병조、병조이침입심부기육조직3개등급대당뇨병성지단배저대서적사지진행평분,계산매지대서적총적분.대이도β세포분비적공능진행평개주요관찰지표:실험전후대서음수량、체질량、삼선감유、담고순、공복혈당、혈지화이도소수평적변화.결과:납입Wistar대서50지,조모불성공탈락20지,30지진입결과분석.①실험기간,모형조화치료조음수량명현고우공백대조조(P<0.01);치료조수착치료시간증가음수량명현하강,모형조수착시간적추이축점승고.②치료후모형조대서체질량명현저우치료전(P<0.01),치료조유하강추세,여치료전비교,차이불명현(P>0.05).③량조대서균유명현지단배저출현(P<0.01).치료조대서적체질량명현고우모형조(P<0.01),지단배저정황명현호우모형조(P<0.01).④치료전치료조화모형조적공복혈당수평명현고우공백대조조(P<0.01),이이도소수평화이도β세포공능지수명현저우공백대조조(P<0.01).치료후량조적공복혈당수평고우공백대조조(P<0.01),치료조적명현저우모형조(P<0.01),이접근정상치;량조혈지화이도β세포공능지수균명현저우공백대조조(P<0.01),치료조명현고우모형조(P<0.01),접근정상치.⑤치료전기타량조삼선감유、담고순수평명현고우공백대조조(P<0.01).경과치료,치료조삼선감유、담고순명현하강(P<0.01),여공백대조조비교,차이불명현(P>0.05);모형조계속승고,명현고우공백대조조(P<0.01).결론:혈청이도소수평승고,혈당、혈지수평하강,가재일정정도상방치당뇨병족적발생、발전.해당뇨병성지단배저모형대약물반응민감,가용우연구당뇨병족발병궤제급약물치료효과적평개.
BACKGROUND:Diabetic acromelic gangrene(diabetic foot)has become one of the important causes for the disability and death in diabetes mellitus.OBJECTIVE:To establish model of diabetic foot in rat,and observe the interventional effect of Chinese compound on diabetic foot.DESIGN:A comparative observational experiment.SETTING:The First Affiliated Hospital of Clinical Medical College,Henan University of Science and Technology.MATERIALS:Fifty male Wistar rats of 8 weeks old,(200.0±16.3)g,were raised with common feed in separate cage at the room temperature of 18-22℃.and they were free to access of feed and water.Ten rats were randomly selected as the blank control group,and the other 40 were used for model establishment.METHODS:The experiments were carried out in the Animal Room of Henan University of Science and Technology from October 2001 to April 2002.①Grouping:The 40 rats were fasted for 6 hours,and then treated with intraperitoneal injection of streptozotocin(55.0 mg/kg),while the 10 rats in the blank control group were injected with isovolume sodium citrate buffer solution.20 models were successfully established,and they were randomly divided into model group(n=10)and treatment group(n=10).Rats in the treatment group were treated for 3 weeks with intragastric perfusion of Chinese compound(60 g/kg)at 9:00 every day after model establishment,and those in the model group were given intragastric perfusion of isovolume saline.At the end of the third week,the rats were all killed under anesthesia after fasted for 12 hours,blood samples were collected to determine the levels of fasting blood glucose,blood lipids and insulin.The daily amount of drinking water was recorded in each group during the experiment.②Scoring standards for acromelic gangrenes:The limbs rats with diabetic foot were scored by three grades,including dark skin,mild open focus on skin,and focus had invaded deep muscular tissue.The total score of each rat was calculated.The beta-cell function index (HBCI)was also evaluated.MAIN OUTCOME MEASURES:The changes of the amount of drinking water,body mass and levels of triglyceride,cholesterol,fasting blood glucose,blood lipids and insulin were observed before and after treatment.RESULTS:Totally 50 Wistar rats were used.20 of them were excluded due to unsuccessful model establishment,and the other 30 rats were involved in the final analysis of results.①The amount of drinking water was obviously higher in the model group and treatment group than in the blank control group during the experiment(P<0.01).As the treatmentlasted,the amount of drinking water was obviously decreased in the treatment group,but gradually increased in the model group.②After treatment,the body mass was obviously lower than that before treatment in the model group(P<0.01).but had a descending trend without obvious difference as compared with that before treatment in the treatment group(P>0.05).③Obvious acromelic gangrenes were obvious in both groups(P<0.01).The body mass in the treatment group was obviously higher than that in the model group(P<0.01),and the conditions of acromelic gangrene were obviously better than those in the model group(P<0.01).④Before treatment,the levels of fasting blood glucose in the treatment group and model group were obviously higher than that in the blank control group(P<0.01),while the levels of insulin and HBCl were obviously lower than those in the blank control group(P<0.01).After treatment,the levels of fasting blood glucose in the treatment group and model group were obviously higher than that in the blank control group(P<0.01),and it was obviously lower in the treatment group than in the model group(P<0.01),it was close to the normal value in the treatment group.⑤The levels of triglyceride and cholesterol before treatment were obviously higher in the treatment group and model group than in the blank control group(P<0.01).After treatment,the levels of triglyceride and cholesterol in the treatment group were obviously decreased(P<0.01), which were not obviously different from those in the blank control group (P>0.05), while those in the model group were increased continuously,and obviously higher than those in the blank control group(P<0.01).CONCLUSION:Increasing the serum level of insulin and decreasing the levels of blood glucose and blood lipids can prevent and treat the occurrence and development of diabetic foot to some extent.This model of diabetic foot is sensitive to drug,and can be used to investigate the pathogenesis of diabetic foot and evaluate the effect of drug therapy.