CAJ | 학술논문

以玉树的茎、叶、根为外植体,研究不同培养基和植物生长调节剂对其再生的影响.结果表明,在附加0.1～10 μmol·L-1 NAA和10 μmol·L-1 BA的MS培养基上,不定芽形成率达到100%,每1个外植体再生芽数达到10个以上.将再生芽转接到含有1 μmol·L-1 NAA或者1 μmol·L-1 IBA的1/2MS培养基上,发育成健壮小植株.生根植株移栽到大田,生长良好,8个月后株高达27 cm.
이옥수적경、협、근위외식체,연구불동배양기화식물생장조절제대기재생적영향.결과표명,재부가0.1～10 μmol·L-1 NAA화10 μmol·L-1 BA적MS배양기상,불정아형성솔체도100%,매1개외식체재생아수체도10개이상.장재생아전접도함유1 μmol·L-1 NAA혹자1 μmol·L-1 IBA적1/2MS배양기상,발육성건장소식주.생근식주이재도대전,생장량호,8개월후주고체27 cm.
Effects of medium and plant growth regulators on shoot regeneration from stem, leaf and root explants of Crassula arborescens were studied. Adventitious buds developed into shoots, shoot regeneration rate reached 100% and shoot number was above 10 per explant on the Murashige and Skoog basal medium (MS) supplemented with 0. 1-10 μmol. L-1 α-naphthaleneacetic acid (NAA) and 10 μmol. L-1 N-6-benzyladenine (BA). Rooted shoots grew into vigorous plantlets after transferred to 1/2 MS medium containing 1 μmol. L-1 NAA or 1 μmol. L-1 indolebutyric acid (IBA). The rooted plantlets were transplanted to the field, grew very vigorous and reached 27 cm height after 8 months.