中药新药与临床药理
中藥新藥與臨床藥理
중약신약여림상약리
TRADITIONAL CHINESE DRUG RESEARCH&CLINICAL PHARMACOLOGY
2009年
4期
354-356
,共3页
天元颗粒%天麻素%HPLC
天元顆粒%天痳素%HPLC
천원과립%천마소%HPLC
Tianyuan Granula%Gastrodin%HPLC
目的 测定天元颗粒中天麻素的含量.方法 样品采用甲醇回流提取,经中性氧化铝柱用80%乙醇洗脱收集;色谱柱:Diamonsil C18柱(250 mm×4.6mm,5μm),流动相:乙腈-0.15%磷酸水溶液(1:99),检测波长:220 nm,流速:1 mL/min,柱温:30℃.结果 天麻素在0.376～2.632 μg之间线性关系良好,r=0.9999 95(n=7),平均回收率99.09%,RSD为1.5%(n=6).结论 该法准确、重复性好,可用于天元颗粒制剂及天麻药材的含量测定及质量控制.
目的 測定天元顆粒中天痳素的含量.方法 樣品採用甲醇迴流提取,經中性氧化鋁柱用80%乙醇洗脫收集;色譜柱:Diamonsil C18柱(250 mm×4.6mm,5μm),流動相:乙腈-0.15%燐痠水溶液(1:99),檢測波長:220 nm,流速:1 mL/min,柱溫:30℃.結果 天痳素在0.376～2.632 μg之間線性關繫良好,r=0.9999 95(n=7),平均迴收率99.09%,RSD為1.5%(n=6).結論 該法準確、重複性好,可用于天元顆粒製劑及天痳藥材的含量測定及質量控製.
목적 측정천원과립중천마소적함량.방법 양품채용갑순회류제취,경중성양화려주용80%을순세탈수집;색보주:Diamonsil C18주(250 mm×4.6mm,5μm),류동상:을정-0.15%린산수용액(1:99),검측파장:220 nm,류속:1 mL/min,주온:30℃.결과 천마소재0.376～2.632 μg지간선성관계량호,r=0.9999 95(n=7),평균회수솔99.09%,RSD위1.5%(n=6).결론 해법준학、중복성호,가용우천원과립제제급천마약재적함량측정급질량공제.
Objective To establish a method for the determination of gastrodin in Tianyuan Granula. Methods The sample was refluxed with methanol, then eluted with 80 % ethanol through the alumina column(neutral). The chromato-graphic conditions were as follows: Diamonsil Cts chromatographic column(250 mm ×4.6 mm, 5 μm) with a mobile phase of acetonitrile-0. 15 % phosphoric acid (1 : 99), the detection wavelength being at 220 nm and the flow rate be-good (r=0.9999 95, n=7).The average recovery was 99.09 % and RSD=1. 50 % (n=6). Conclusion The method for determination of gastrodin in Tianyuan Granula is accurate, and with a good reproducibility.
