CAJ | 학술논문

选用4头体重为559.4±30.1 kg,健康且装有瘤胃瘘管的4岁龄杂交肉牛,进行重复交叉试验设计,处理组添加维吉尼亚霉素,旨在观察日粮中添加维基尼亚霉素对瘤胃微生物数量的影响.在每个正试验期第28天的饲喂前8:00和饲喂后12:00、16:00采集瘤胃液进行亨氏滚管法培养计数.试验发现日粮中添加维基尼亚霉素能有效抑制调节瘤胃微生物中淀粉降解菌数量和蛋白降解菌数量(P<0.01),但对纤维降解菌、总细菌和原虫的数量影响不显著.维吉尼亚霉素在不同采样时间点抑制调节瘤胃微生物数量的统计结果显示,维吉尼亚霉素在8:00时,能有效抑制调节淀粉降解菌数量(P<0.05),在12:00时,能有效抑制淀粉降解菌数量和蛋白降解菌数量(P<0.05).维吉尼亚霉素在不同时间点对纤维降解菌、总细菌和原虫的数量影响不显著.但对照组和处理组中,原虫数量和总细菌数量随时间均呈现出"高-低-高"的趋势.结果表明,向日粮中添加维吉尼亚霉素能有效调节高精料条件下占主导地位的淀粉降解菌和蛋白降解菌的数量,从而缓解瘤胃酸中毒.
선용4두체중위559.4±30.1 kg,건강차장유류위루관적4세령잡교육우,진행중복교차시험설계,처리조첨가유길니아매소,지재관찰일량중첨가유기니아매소대류위미생물수량적영향.재매개정시험기제28천적사위전8:00화사위후12:00、16:00채집류위액진행형씨곤관법배양계수.시험발현일량중첨가유기니아매소능유효억제조절류위미생물중정분강해균수량화단백강해균수량(P<0.01),단대섬유강해균、총세균화원충적수량영향불현저.유길니아매소재불동채양시간점억제조절류위미생물수량적통계결과현시,유길니아매소재8:00시,능유효억제조절정분강해균수량(P<0.05),재12:00시,능유효억제정분강해균수량화단백강해균수량(P<0.05).유길니아매소재불동시간점대섬유강해균、총세균화원충적수량영향불현저.단대조조화처리조중,원충수량화총세균수량수시간균정현출"고-저-고"적추세.결과표명,향일량중첨가유길니아매소능유효조절고정료조건하점주도지위적정분강해균화단백강해균적수량,종이완해류위산중독.
This experiment was conducted to investigate the influence of adding virginiamycin to diets of steers on ruminal microbial populations. Four ruminally cannulated steers (559.4±30.1 kg) were utilized in a repeat crossover design. Treatment group added virginiamycin. Ruminal fluid was collected on 28 d of experimental period, starting at 8:00 prefeeding and at 12:00 and 16:00 post-feeding from the anterior, dorsal, and mid-ventral region of the rumen. The Amount of microbial was counted after culturing using roll-tube technique. We detected that adding virginiamycin to diets of steers could active suppression and regulate amylolytic bacteria populations and proteolytic bacteria populations (P<0.01). But the populations of cellulolytic bacteria, total viable bacteria and protozoon were no difference between control group and treatment group. Statistics result of virginiamycin regulated that the different sampling time showed: viginiamycin could inhibite and regulate amylolytic bacteria populations when the sampling time was at 8:00 (P<0.05). Viginiamycin could inhibite and regulate amylolytic bacteria populations and proteolytic bacteria populations when the sampling time was at 12: 00(P<0.05). But the populations of cellulolytic bacteria, total viable bacteria and protozoon were no difference between control group and treatment group at the different sampling time. But the populations of total viable bacteria and protozoon to showed a tendency that "high-low-high" with time changed between control group and treatment group. The results showed that virginiamycin could active suppression and regulate amylolytic bacteria populations and proteolytic bacteria populations on high concentration. Therefore, virginiamycin potentially could prevent ruminal acidosis.