解剖学报
解剖學報
해부학보
ACTA ANATOMICA SINICA
2009年
4期
555-559
,共5页
尼古丁%帕金森病%脂多糖%小胶质细胞%多巴胺%免疫印迹法%反转录-聚合酶链式反应%PD大鼠
尼古丁%帕金森病%脂多糖%小膠質細胞%多巴胺%免疫印跡法%反轉錄-聚閤酶鏈式反應%PD大鼠
니고정%파금삼병%지다당%소효질세포%다파알%면역인적법%반전록-취합매련식반응%PD대서
Nicotine%Parkinson's disease%Lipopolysaccharide%Microglia%Dopamine%Western blotting%RT-PCR%PD Rat
目的 探讨尼古丁对帕金森病(PD)大鼠黑质多巴胺能神经元变性的影响及其机制. 方法 45只大鼠随机分为PBS对照组(CON)、生理盐水+ 脂多糖(NS)组、尼古丁+脂多糖(NIC)组,每组15只.黑质内立体定向注射脂多糖(LPS)或PBS后24h,免疫印迹法检测黑质诱导性一氧化氮合酶(iNOS)蛋白表达变化;黑质注射药物后14d,采用免疫组织化学法观察大鼠黑质酪氨酸羟化酶(TH)阳性神经元数量及OX-42阳性细胞形态学变化,RT-PCR及免疫印迹检测黑质TH mRNA及TH蛋白的表达水平. 结果 与CON组相比,NS组大鼠黑质iNOS表达明显增多,TH阳性神经元、TH mRNA及TH蛋白明显减少,小胶质细胞大多呈胞体大突起短粗的形态;NIC组黑质iNOS表达明显少于NS组,黑质TH阳性神经元、TH mRNA及TH蛋白表达较NS组明显增多,大部分小胶质细胞呈胞体小,突起细长的形态. 结论 尼古丁可以减轻LPS介导的多巴胺能神经元变性,对多巴胺能神经元有保护作用,其保护机制与抑制小胶质细胞激活、减少iNOS的表达有关.
目的 探討尼古丁對帕金森病(PD)大鼠黑質多巴胺能神經元變性的影響及其機製. 方法 45隻大鼠隨機分為PBS對照組(CON)、生理鹽水+ 脂多糖(NS)組、尼古丁+脂多糖(NIC)組,每組15隻.黑質內立體定嚮註射脂多糖(LPS)或PBS後24h,免疫印跡法檢測黑質誘導性一氧化氮閤酶(iNOS)蛋白錶達變化;黑質註射藥物後14d,採用免疫組織化學法觀察大鼠黑質酪氨痠羥化酶(TH)暘性神經元數量及OX-42暘性細胞形態學變化,RT-PCR及免疫印跡檢測黑質TH mRNA及TH蛋白的錶達水平. 結果 與CON組相比,NS組大鼠黑質iNOS錶達明顯增多,TH暘性神經元、TH mRNA及TH蛋白明顯減少,小膠質細胞大多呈胞體大突起短粗的形態;NIC組黑質iNOS錶達明顯少于NS組,黑質TH暘性神經元、TH mRNA及TH蛋白錶達較NS組明顯增多,大部分小膠質細胞呈胞體小,突起細長的形態. 結論 尼古丁可以減輕LPS介導的多巴胺能神經元變性,對多巴胺能神經元有保護作用,其保護機製與抑製小膠質細胞激活、減少iNOS的錶達有關.
목적 탐토니고정대파금삼병(PD)대서흑질다파알능신경원변성적영향급기궤제. 방법 45지대서수궤분위PBS대조조(CON)、생리염수+ 지다당(NS)조、니고정+지다당(NIC)조,매조15지.흑질내입체정향주사지다당(LPS)혹PBS후24h,면역인적법검측흑질유도성일양화담합매(iNOS)단백표체변화;흑질주사약물후14d,채용면역조직화학법관찰대서흑질락안산간화매(TH)양성신경원수량급OX-42양성세포형태학변화,RT-PCR급면역인적검측흑질TH mRNA급TH단백적표체수평. 결과 여CON조상비,NS조대서흑질iNOS표체명현증다,TH양성신경원、TH mRNA급TH단백명현감소,소효질세포대다정포체대돌기단조적형태;NIC조흑질iNOS표체명현소우NS조,흑질TH양성신경원、TH mRNA급TH단백표체교NS조명현증다,대부분소효질세포정포체소,돌기세장적형태. 결론 니고정가이감경LPS개도적다파알능신경원변성,대다파알능신경원유보호작용,기보호궤제여억제소효질세포격활、감소iNOS적표체유관.
Objective To explore the effect of nicotine on the degeneration of dopaminergic neurons in PD rats. Methods Forty-five SD rats were randomly divided into three groups: PBS group (CON), normal saline + LPS ( NS ) group and nicotine +LPS ( NIC ) group. On 24 hours after LPS or PBS injection, inducible nitric oxide synthase ( iNOS ) protein expression was examined by immunoblotting. ON 14d after LPS or PBS injection, the numbers of tyrosine hydroxylase ( TH ) positive neurons and morphological changes of OX-42 positive cells in the substantia nigra(SN) were observed by immunohistochemistry. TH mRNA and TH protein expressions were examined by RT-PCR or immunoblotting. Results Compared with CON group, iNOS protein increased markedly 24 hours after LPS injection in NS group. TH positive neurons, TH mRNA and TH protein in the SN decreased remarkably 14 days after nigrainjiection. Most of microlglial showed big cell body with thicker and shorter processes. However, nicotine reversed the above changes, Compared with NS group, TH positive neurons, TH mRNA and TH protein in the SN increased remarkably in NIC. Besides, most microglia showed small cell body with slim and long processes. Conclusion Nicotine could prevent LPS-induced degeneration of DA neurons, probably because of that the pretreatment with nicotine blocks the activation of microglia and the expression iNOS protein.
