CAJ | 학술논문

利用乳酸杆菌分离培养基(SL)对犊牛瘤胃内的乳酸杆菌进行分离,对分离出的乳酸杆菌进行革兰氏染色、生化反应及16S rRNA同源性分析鉴定.对分离鉴定的干酪乳杆菌提取其基因组,根据Genbank 发表的干酪乳杆菌耐酸相关基因(ffh基因)设计1对引物进行PCR,利用pMD18-T载体进行克隆,构建重组质粒,进行PCR及测序鉴定,获得耐酸基因ffh,为下一步研制瘤胃微生态制剂及构建瘤胃内耐酸的乳酸分解基因工程菌奠定了基础.
이용유산간균분리배양기(SL)대독우류위내적유산간균진행분리,대분리출적유산간균진행혁란씨염색、생화반응급16S rRNA동원성분석감정.대분리감정적간락유간균제취기기인조,근거Genbank 발표적간락유간균내산상관기인(ffh기인)설계1대인물진행PCR,이용pMD18-T재체진행극륭,구건중조질립,진행PCR급측서감정,획득내산기인ffh,위하일보연제류위미생태제제급구건류위내내산적유산분해기인공정균전정료기출.
The SL medium was used to isolate Lactobacillus casei. The Gram staining, biochemistry I-dentification, morphology observation and 16S rRNA phylogenelic analysis were used to identify the bacteria. The genome of the identified Lactobacillus casei was extracted, and according to gene ffh from GenBank, a pair of primers were designed for PCR, then the gene was cloned into pMD 18-T vector to obtain the recombinant plasmid and detected by PCH and homology sequence analysis. The whole sequence of gene ffh was acquired. This work made preparation for the study of rumen microbial ecological agents and the acid-resistant genetic engineering degradation bacteria of lactic acid.