CAJ | 학술논문

药用野生稻是中国最具利用价值的野生稻资源之一,具有许多优良的性状和有利基因,是改良栽培稻培育新品种的宝贵基因库,而其CC基因组的优良基因有望以大片段形式通过BIBAC等载体转化到栽培稻中.BIBAC文库不仅能作为大片段基因组文库的载体,而且能通过根癌农杆菌介导将克隆片段导入植物基因组直接进行转化,可用于筛选分离基因等研究.如何获得基因组DNA大片段非常关键,本研究采取琼脂糖包埋基因组的方法获得大片段DNA,并对其中的一些步骤进行了优化,为构建药用野生稻BIBAC文库打下了很好的基础,值得在其它植物类似研究中参考.
약용야생도시중국최구이용개치적야생도자원지일,구유허다우량적성상화유리기인,시개량재배도배육신품충적보귀기인고,이기CC기인조적우량기인유망이대편단형식통과BIBAC등재체전화도재배도중.BIBAC문고불부능작위대편단기인조문고적재체,이차능통과근암농간균개도장극륭편단도입식물기인조직접진행전화,가용우사선분리기인등연구.여하획득기인조DNA대편단비상관건,본연구채취 경지당포매기인조적방법획득대편단DNA,병대기중적일사보취진행료우화,위구건약용야생도BIBAC문고타하료흔호적기출,치득재기타식물유사연구중삼고.
Oryza officinalis Wall.ex Watt is one of the most valuable wild rice resources in China. It contains a lot of specific and helpful genes for the further improvement of cultivated rice. Some excellent genes existing in O. officinalis were possibly transferred into some cultivated rice through BIBAC (binary bacterial artificial chromosome,BIBAC) vector in the form of large fragments. BIBAC vector was one of the efficient vectors in gene cloning and transformation in plants. The key to construct genome library was to obtain mega-fragments. The way that mega-fragments were embedded in agarose gel was used and optimized in this study,which could provide a solid foundation to construct the BIBAC library of O. officinalis.