上海农业学报
上海農業學報
상해농업학보
ACTA AGRICULTURAE SHANGHAI
2010年
1期
129-132
,共4页
赵杰%庄隽怡%唐亚芹%顾振芳
趙傑%莊雋怡%唐亞芹%顧振芳
조걸%장준이%당아근%고진방
甜瓜枯萎病菌%生物学特性%生化特性%抑制作用%杀菌剂筛选
甜瓜枯萎病菌%生物學特性%生化特性%抑製作用%殺菌劑篩選
첨과고위병균%생물학특성%생화특성%억제작용%살균제사선
Fusarium oxysporum(Schl.)f.sp.melonis%Biological characteristics%Biochemical characteristics%Inhibiting effect%Screening of Fungicide
研究了甜瓜枯萎病菌的生物学特性,并对防治药剂进行了室内初步筛选.结果表明:菌丝生长的适宜温度范围为5~35 ℃,最适温度30 ℃,菌丝和孢子的致死温度为58 ℃;光暗交替条件下产孢量最大.生理生化特性测定结果表明:甜瓜枯萎病菌对孔雀石绿不敏感,对淀粉和硝态氮的利用能力强.采用生长速率法测定了9种杀菌剂对病菌的抑制作用,其中多菌灵和氟硅唑的毒力较高,EC_(50)分别为2.2671 mg/L和13.3418 mg/L.
研究瞭甜瓜枯萎病菌的生物學特性,併對防治藥劑進行瞭室內初步篩選.結果錶明:菌絲生長的適宜溫度範圍為5~35 ℃,最適溫度30 ℃,菌絲和孢子的緻死溫度為58 ℃;光暗交替條件下產孢量最大.生理生化特性測定結果錶明:甜瓜枯萎病菌對孔雀石綠不敏感,對澱粉和硝態氮的利用能力彊.採用生長速率法測定瞭9種殺菌劑對病菌的抑製作用,其中多菌靈和氟硅唑的毒力較高,EC_(50)分彆為2.2671 mg/L和13.3418 mg/L.
연구료첨과고위병균적생물학특성,병대방치약제진행료실내초보사선.결과표명:균사생장적괄의온도범위위5~35 ℃,최괄온도30 ℃,균사화포자적치사온도위58 ℃;광암교체조건하산포량최대.생리생화특성측정결과표명:첨과고위병균대공작석록불민감,대정분화초태담적이용능력강.채용생장속솔법측정료9충살균제대병균적억제작용,기중다균령화불규서적독력교고,EC_(50)분별위2.2671 mg/L화13.3418 mg/L.
The biological characteristics of muskmelon fusarium pathogen were studied and the preliminary screening of fungicides was conducted in a laboratory.The results showed that the suitable temperature for the mycelial growth was 5~35 ℃ and the best was 30 ℃.The thermal death temperature of the mycelia and conidia was 58 ℃.Alternation of light and dark could promote the spore production.The tests for the biochemical characteristics showed that Fusarium oxysporum(Schl.)f.sp.melonis was insensitive to malachite green and could utilize starch and nitrate nitrogen.The tests for the inhibiting effects of 9 fungicides on F.oxysporum f.sp.melonis indicated that carbendazim and flusilazole were the highest in toxicity and their EC_(50) was 2.2671 mg/L and 13.3418 mg/L,respectively.