河北化工
河北化工
하북화공
HEBEI CHEMICAL ENGINEERING AND INDUSTRY
2011年
9期
59-60,68
,共3页
盐酸萘乙二胺%光度法%奶制品%亚硝酸盐
鹽痠萘乙二胺%光度法%奶製品%亞硝痠鹽
염산내을이알%광도법%내제품%아초산염
hydrochloric acid naphthalene ethylenediamine%spectrophotometry method%milk products%nitrite
采用盐酸萘乙二胺分光光度法对奶制品中的亚硝酸盐含量进行了测定。系统考察了重氮化试剂和显色剂用量、重氮化反应和显色反应的反应时间等对测定灵敏度的影响,并优化了仪器的测量条件。实验结果表明,在最佳实验条件下,以λ=540 nm为测定波长,亚硝酸盐在0～0.2μg/L内线性良好,相关系数为0.999 7。奶制品样品加标回收率在100%～105%之间。该方法灵敏度高、操作简便,值得推广。
採用鹽痠萘乙二胺分光光度法對奶製品中的亞硝痠鹽含量進行瞭測定。繫統攷察瞭重氮化試劑和顯色劑用量、重氮化反應和顯色反應的反應時間等對測定靈敏度的影響,併優化瞭儀器的測量條件。實驗結果錶明,在最佳實驗條件下,以λ=540 nm為測定波長,亞硝痠鹽在0～0.2μg/L內線性良好,相關繫數為0.999 7。奶製品樣品加標迴收率在100%～105%之間。該方法靈敏度高、操作簡便,值得推廣。
채용염산내을이알분광광도법대내제품중적아초산염함량진행료측정。계통고찰료중담화시제화현색제용량、중담화반응화현색반응적반응시간등대측정령민도적영향,병우화료의기적측량조건。실험결과표명,재최가실험조건하,이λ=540 nm위측정파장,아초산염재0～0.2μg/L내선성량호,상관계수위0.999 7。내제품양품가표회수솔재100%～105%지간。해방법령민도고、조작간편,치득추엄。
The content of nitrite in milk products was determined by spectrophotometry method with hydrochloric acid naphthalene ethylenediamine as the color reaction reagent.The factors that affected the sensitivity were investigated,in-cluding the quantity of diazotization reagent and color reaction reagent,the time of diazotization reaction and color reac-tion.Under the optimized conditions,the linearity was ranged in 0～0.2 μg/L（r =0.999 7） with the detection wavelength of 540 nm.The spiked recoveries were ranged from 100 % to 122.8%.The poposed method had the characters of high sensitivity,simple operation and worth application.
