CAJ | 학술논문

隐孢子虫是一种属于顶复门的寄生性肠道原虫,主要寄生于人或动物的消化道上皮并引起腹泻,对公共卫生造成很大威胁.随着近年来分子生物学的迅猛发展,越来越多的基因分型技术因其分辨率、敏感性及特异性较高而被广泛应用于隐孢子虫研究中,进而为追溯传染源、调查传播途径及制定有效干预举措提供重要理论依据.该文就几种主要基于PeR的隐孢子虫基因分型技术的研究进展作一简要综述.
은포자충시일충속우정복문적기생성장도원충,주요기생우인혹동물적소화도상피병인기복사,대공공위생조성흔대위협.수착근년래분자생물학적신맹발전,월래월다적기인분형기술인기분변솔、민감성급특이성교고이피엄범응용우은포자충연구중,진이위추소전염원、조사전파도경급제정유효간예거조제공중요이론의거.해문취궤충주요기우PeR적은포자충기인분형기술적연구진전작일간요종술.
Cryptosporidium,a protozoan of Apicomplexan that invades the gastrointestinal epithelium of humans and a wide variety of animals, causes self-limiting diarrhea but sometimes severe diarrhea in immuno-competent humans thus to pose a significant public health risk. Recently with the development of molecular bi-ology, more and more molecular techniques have been used in genotyping Cryptosporidium due to their high res-olution, sensitivity and specificity. These tools will play an important role in tracking infection sources,charac-terizing the transmission dynamics, and making effective intervention strategies for public health. This review summarized the advances of some PCR-based techniques mainly used in Cryptosporidium genotyping. [ Key words ] Cryptosporidium; Genotyping techniques ; Principle; Application