CAJ | 학술논문

目的探讨支气管哮喘患者诱导痰中白细胞介素-25(IL-25)、嗜酸性粒细胞(EOS)在支气管哮喘发病中的作用.方法选择急性发作期支气管哮喘患者30例(哮喘组)、其中经治疗后23例患者病情缓解(缓解组)、同期健康体检者20例作为对照组,均采用超声雾化吸入梯度高渗盐水法采集诱导痰标本,以ELISA法测定诱导痰中IL-25水平,计数EOS,并分析两者相关性.结果哮喘组诱导痰中IL-25及EOS水平均显著高于对照组[(313.12±75.64)ng/L与(236.43±57.90) ng/L、(0.386±0.267)×109/L与(0.005±0.002)×109/L,t=16.77、19.28,均P＜0.05],且急性发作期显著高于缓解期[(268.63±40.19) ng/L、(0.120±0.016)×109/L](t=11.65、12.37,均P＜0.05).结论 IL-25、EOS在哮喘发生、发展中具有重要作用,为监测和治疗提供了一种新的思路.
목적 탐토지기관효천환자유도담중백세포개소-25(IL-25)、기산성립세포(EOS)재지기관효천발병중적작용.방법선택급성발작기지기관효천환자30례(효천조)、기중경치료후23례환자병정완해(완해조)、동기건강체검자20례작위대조조,균채용초성무화흡입제도고삼염수법채집유도담표본,이ELISA법측정유도담중IL-25수평,계수EOS,병분석량자상관성.결과 효천조유도담중IL-25급EOS수평균현저고우대조조[(313.12±75.64)ng/L여(236.43±57.90) ng/L、(0.386±0.267)×109/L여(0.005±0.002)×109/L,t=16.77、19.28,균P＜0.05],차급성발작기현저고우완해기[(268.63±40.19) ng/L、(0.120±0.016)×109/L](t=11.65、12.37,균P＜0.05).결론 IL-25、EOS재효천발생、발전중구유중요작용,위감측화치료제공료일충신적사로.
Objective To investigate the roles of interleukin(IL)-25,eosinophils in the occurrence of bronchial asthma.Methods Selected 30 cases of acute episode of asthma(asthma group),and ease a group of 23 cases of asthma treatment(remission group),20 cases of the normal control group.Using the method of gradient ultrasonic atomizing inhalation of Hyperosmotic saline collection of induced sputum specimens,by ELISA method for the determination IL-25 level in induced sputum,count of eosinophils,analysis of correlation between them.Results Group IL-25 in induced sputum of asthma and eosinophil levels[(313.12±75.64)ng/L vs(236.43±57.90)ng/L]were sig-nificandy higher than the control group[(0.386±0.267) × 109/L vs(0.005±0.002) × 109/L],and significantly higher than acute stage in remission stage[(268.63 + 40.19) ng/L,(0.120 + 0.016) × 109/L](P ＜ 0.05).Conclusion IL-25 has an important role in asthma development.It provides a new way of thinking for monitoring and treatment.