中华风湿病学杂志
中華風濕病學雜誌
중화풍습병학잡지
CHINESE JOURNAL OF RHEUMATOLOGY
2011年
10期
710-712
,共3页
郝亚荣%陈宣银%邱波%汪喆%周建林
郝亞榮%陳宣銀%邱波%汪喆%週建林
학아영%진선은%구파%왕철%주건림
血管内皮生长因子类%骨关节炎%软骨细胞%胶原Ⅱ型
血管內皮生長因子類%骨關節炎%軟骨細胞%膠原Ⅱ型
혈관내피생장인자류%골관절염%연골세포%효원Ⅱ형
Vascular endothelialgrowthfactors%Osteoarthritis%Chondrocytes%Collagen type Ⅱ
目的 观察血管内皮生长因子(VEGF)对关节软骨细胞Ⅱ型胶原表达的影响.方法 体外培养乳鼠关节软骨细胞,实验分为4组,加入不同因素干预.A组(对照组):不加处理因素；B组:10 ng/mlVEGF;C组:10 ng/ml白细胞介素(IL)-1β;D组:10 ng/ml VEGF+10 ng/ml IL-1β.采用实时荧光定量聚合酶链反应检测Ⅱ型胶原mRNA的表达,采用蛋白免疫印迹法检测Ⅱ型胶原蛋白的表达.采用单因素方差分析进行统计学处理.结果 各组软骨细胞Ⅱ型胶原mRNA的相对表达量分别为:A组1.00±0.08,B组0.78±0.07,C组0.67±0.06,D组0.57±0.04,B、C及D组软骨细胞Ⅱ型胶原的mRNA表达量均显著低于A组(P均＜0.01),D组Ⅱ型胶原的mRNA表达水平明显低于B组及C组；与A组(0.95±0.21)比较,B组(0.7 1±.0.14)、C组(0.60±0.11)及D组(0.31±0.09)的Ⅱ型胶原蛋白表达量显著降低(P均＜0.01),D组软骨细胞Ⅱ型胶原的蛋白表达水平明显低于B组及C组(P均＜0.01).结论 在骨关节炎的发病过程中,VEGF可能通过抑制关节软骨细胞Ⅱ型胶原的表达发挥重要作用.
目的 觀察血管內皮生長因子(VEGF)對關節軟骨細胞Ⅱ型膠原錶達的影響.方法 體外培養乳鼠關節軟骨細胞,實驗分為4組,加入不同因素榦預.A組(對照組):不加處理因素；B組:10 ng/mlVEGF;C組:10 ng/ml白細胞介素(IL)-1β;D組:10 ng/ml VEGF+10 ng/ml IL-1β.採用實時熒光定量聚閤酶鏈反應檢測Ⅱ型膠原mRNA的錶達,採用蛋白免疫印跡法檢測Ⅱ型膠原蛋白的錶達.採用單因素方差分析進行統計學處理.結果 各組軟骨細胞Ⅱ型膠原mRNA的相對錶達量分彆為:A組1.00±0.08,B組0.78±0.07,C組0.67±0.06,D組0.57±0.04,B、C及D組軟骨細胞Ⅱ型膠原的mRNA錶達量均顯著低于A組(P均＜0.01),D組Ⅱ型膠原的mRNA錶達水平明顯低于B組及C組；與A組(0.95±0.21)比較,B組(0.7 1±.0.14)、C組(0.60±0.11)及D組(0.31±0.09)的Ⅱ型膠原蛋白錶達量顯著降低(P均＜0.01),D組軟骨細胞Ⅱ型膠原的蛋白錶達水平明顯低于B組及C組(P均＜0.01).結論 在骨關節炎的髮病過程中,VEGF可能通過抑製關節軟骨細胞Ⅱ型膠原的錶達髮揮重要作用.
목적 관찰혈관내피생장인자(VEGF)대관절연골세포Ⅱ형효원표체적영향.방법 체외배양유서관절연골세포,실험분위4조,가입불동인소간예.A조(대조조):불가처리인소；B조:10 ng/mlVEGF;C조:10 ng/ml백세포개소(IL)-1β;D조:10 ng/ml VEGF+10 ng/ml IL-1β.채용실시형광정량취합매련반응검측Ⅱ형효원mRNA적표체,채용단백면역인적법검측Ⅱ형효원단백적표체.채용단인소방차분석진행통계학처리.결과 각조연골세포Ⅱ형효원mRNA적상대표체량분별위:A조1.00±0.08,B조0.78±0.07,C조0.67±0.06,D조0.57±0.04,B、C급D조연골세포Ⅱ형효원적mRNA표체량균현저저우A조(P균＜0.01),D조Ⅱ형효원적mRNA표체수평명현저우B조급C조；여A조(0.95±0.21)비교,B조(0.7 1±.0.14)、C조(0.60±0.11)급D조(0.31±0.09)적Ⅱ형효원단백표체량현저강저(P균＜0.01),D조연골세포Ⅱ형효원적단백표체수평명현저우B조급C조(P균＜0.01).결론 재골관절염적발병과정중,VEGF가능통과억제관절연골세포Ⅱ형효원적표체발휘중요작용.
Objective To investigate the effect of vascular endothelial growth factor (VEGF) on collagen Ⅱ expression in rat articular chondrocytes in vitro.Methods Chondrocytes were isolated and cultured.Then rats were divided into 4 groups:group A (control):without any intervention; group B:10 ng/ml VEGF was added; group C:10 ng/ml IL-1β was added; group D:10 ng/ml VEGF and 10 ng/ml IL-1β were added.Messenger RNA (mRNA) expression of collagen Ⅱ was detected by using real time polymerase chain reaction (real time PCR),and the protein expression level of collagen Ⅱ was detected by Western blotting.Comparisons between groups were performed by one-way ANOVA.Results The collagen Ⅱ mRNA expression levels of group B (0.78+0.07),group C (0.67+0.06) and group D (0.57+0.04) were significantly lower than those of the group A (1.00±0.08),and there was significant difference between B and D,C and D.Compared with group A (0.95+0.21),the expression of collagen Ⅱ protein in group B (0.71+0.14),group C (0.60±0.11) and group D (0.31 +0.09) was significantly suppressed.The expression of collagen Ⅱ protein in group D was significantly lower than those of group B and C.Conclusion VEGF can significantly suppress the expression of collagen II in rat articular chondrocytes.VEGF may play an important role in the development of osteoarthritis.
