CAJ | 학술논문

目的探讨石蜡包埋子宫内膜样腺癌组织中雌激素硫酸转移酶(estrogen sulfotransferase,EST)和甾体硫酸酯酶(steroid sulfatase,STS)的RNA表达. 方法使用罗氏产品从30例石蜡包埋子宫内膜组织(对照组和患病组各15例)中提取EST、STS的RNA. 结果对照组RNA的表达EST为0.25±0.03,STS为0.08±0.02,STS与EST比值为0.11±0.08;患病组RNA的表达:EST为0.06±0.02,STS为0.24±0.92,STS与EST比值4.40±0.64,患病组与对照组比较,差异有统计学意义(t分别为-8.223、7.345和11.591,均P<0.05). 结论 (1)石蜡包埋组织是子宫内膜样腺癌基础研究的材料来源;(2)在子宫内膜样腺癌中,EST下降,STS和STS与EST比值升高.
목적 탐토석사포매자궁내막양선암조직중자격소류산전이매(estrogen sulfotransferase,EST)화치체류산지매(steroid sulfatase,STS)적RNA표체. 방법 사용라씨산품종30례석사포매자궁내막조직(대조조화환병조각15례)중제취EST、STS적RNA. 결과 대조조RNA적표체EST위0.25±0.03,STS위0.08±0.02,STS여EST비치위0.11±0.08;환병조RNA적표체:EST위0.06±0.02,STS위0.24±0.92,STS여EST비치4.40±0.64,환병조여대조조비교,차이유통계학의의(t분별위-8.223、7.345화11.591,균P<0.05). 결론 (1)석사포매조직시자궁내막양선암기출연구적재료래원;(2)재자궁내막양선암중,EST하강,STS화STS여EST비치승고.
Objective To explore the expression of estrogen sulfotransferase(EST) and steroid sulfatase (STS) in formalin-fixed paraffin-embedded endometrioid adenocarcinoma samples. Methods The RNA of EST and STS in 30 formalin-fixed paraffin-embedded endometrium samples were extracted using Roche products. Results The RNA expression of EST and STS were 0.25±0.03 and 0.08±0.02 respectively and the STS/EST was 0.11±0.08 in normal endometrium. While in endometrioid adenocarcinoma the RNA expression of EST and STS were 0.06±0.02 and 0.24±0.92 respectively and the STS/EST was 4.40±0.64. There were significant differences between these two groups. Conclusions (1) Formalin-fixed paraffin-embedded tissues could be used to study the endometrioid adenocarcinoma. (2) EST is decreased, STS and STS/EST are increased in human endometrioid adenocarcinoma. STS/EST may be related with the prognosis of the endometrioid adenocarcinoma.