中国综合临床
中國綜閤臨床
중국종합림상
CLINICAL MEDICINE OF CHINA
2011年
11期
1130-1133
,共4页
孙冬梅%薛瑞凤%郭淑香%唐蕊%李静%谢芳%张树华%刘洋%孙萌%李会学
孫鼕梅%薛瑞鳳%郭淑香%唐蕊%李靜%謝芳%張樹華%劉洋%孫萌%李會學
손동매%설서봉%곽숙향%당예%리정%사방%장수화%류양%손맹%리회학
超声心动图%糖尿病大鼠%心肌病变%左心室功能%超微结构%嗜银纤维
超聲心動圖%糖尿病大鼠%心肌病變%左心室功能%超微結構%嗜銀纖維
초성심동도%당뇨병대서%심기병변%좌심실공능%초미결구%기은섬유
Echocardiography%Diabetic rat%Myocardial disease%Left ventricular diastolic function%Ultrastructure%Argyrophilic fiber
目的 探讨糖尿病大鼠病变过程中(第4、12、24周)左心室舒张功能与心肌超微结构及嗜银纤维动态改变之间的关系.方法 通过一次性大剂量腹腔注射链脲佐菌素(STZ)(65 m/kg)建立雄性SD大鼠糖尿病模型,脉冲多普勒(CDFI-PW)及多普勒组织成像(DTI)技术检测大鼠左心室舒张功能;透射电镜观察心肌超微结构,嗜银染色观察心肌嗜银纤维容积分数(APFVF);并与同龄正常大鼠组进行比较.结果(1)糖尿病组舒张功能减低最早出现在模型成功后第4周(E/A＜1),并逐渐加重,24周达二尖瓣血流假性正常阶段(E/A＞1),DTI可帮助鉴别(Ea/Aa＜1).正常组未见舒张功能异常(E/A＞1和Ea/Aa＞1).(2)透射电镜观察到糖尿病组大鼠心肌细胞的亚细胞重构,表现为肌原纤维含量减少、排列紊乱,线粒体变性,肌浆网扩张、结构紊乱等改变.糖尿病大鼠组心肌APFVF均明显增高(P＜0.05).结论 糖尿病大鼠左心室舒张功能减低与超微结构改变、心肌嗜银纤维增多密切相关.
目的 探討糖尿病大鼠病變過程中(第4、12、24週)左心室舒張功能與心肌超微結構及嗜銀纖維動態改變之間的關繫.方法 通過一次性大劑量腹腔註射鏈脲佐菌素(STZ)(65 m/kg)建立雄性SD大鼠糖尿病模型,脈遲多普勒(CDFI-PW)及多普勒組織成像(DTI)技術檢測大鼠左心室舒張功能;透射電鏡觀察心肌超微結構,嗜銀染色觀察心肌嗜銀纖維容積分數(APFVF);併與同齡正常大鼠組進行比較.結果(1)糖尿病組舒張功能減低最早齣現在模型成功後第4週(E/A＜1),併逐漸加重,24週達二尖瓣血流假性正常階段(E/A＞1),DTI可幫助鑒彆(Ea/Aa＜1).正常組未見舒張功能異常(E/A＞1和Ea/Aa＞1).(2)透射電鏡觀察到糖尿病組大鼠心肌細胞的亞細胞重構,錶現為肌原纖維含量減少、排列紊亂,線粒體變性,肌漿網擴張、結構紊亂等改變.糖尿病大鼠組心肌APFVF均明顯增高(P＜0.05).結論 糖尿病大鼠左心室舒張功能減低與超微結構改變、心肌嗜銀纖維增多密切相關.
목적 탐토당뇨병대서병변과정중(제4、12、24주)좌심실서장공능여심기초미결구급기은섬유동태개변지간적관계.방법 통과일차성대제량복강주사련뇨좌균소(STZ)(65 m/kg)건립웅성SD대서당뇨병모형,맥충다보륵(CDFI-PW)급다보륵조직성상(DTI)기술검측대서좌심실서장공능;투사전경관찰심기초미결구,기은염색관찰심기기은섬유용적분수(APFVF);병여동령정상대서조진행비교.결과(1)당뇨병조서장공능감저최조출현재모형성공후제4주(E/A＜1),병축점가중,24주체이첨판혈류가성정상계단(E/A＞1),DTI가방조감별(Ea/Aa＜1).정상조미견서장공능이상(E/A＞1화Ea/Aa＞1).(2)투사전경관찰도당뇨병조대서심기세포적아세포중구,표현위기원섬유함량감소、배렬문란,선립체변성,기장망확장、결구문란등개변.당뇨병대서조심기APFVF균명현증고(P＜0.05).결론 당뇨병대서좌심실서장공능감저여초미결구개변、심기기은섬유증다밀절상관.
Objective To explore the relationship between left ventricular diastolic function and the dynamic changes of myocardial ultrastructure and argyrophilic fiber in diabetic rats on the different periods of lesions(week 4,12 and 24).Methods The diabetes mellitus(DM)in healthy male Sprague-Dawley rats was induced by a single injection of streptozotocin(STZ,Sigma)into intraperitoneal at a dose of 65 mg/kg body weight.The left ventricular diastolic function was measured by Color Doppler Flow Imaging-Pulsed Wave(CDFIPW)and Doppler Tissue Imaging(DTI)echocardiography.Heart tissue at the apex was obtained rapidly for transmission electron microscope study.Argyrophilic staining was used in the study of argyrophilic fiber volume fraction(APFVF)in heart interstitial tissue.Results Diastolic dysfunction of left ventricle was detected in STZinduced diabetic rats by CDFI-PW(E/A ＜ 1)at week 4,and progressed gradually.Pseudonormal filling (E/A ＞ 1)was found in diabetic rats at week 24,which could be identified by DTI(Ea/Aa ＜ 1).Diastolic function of normal rats was not impaired(E/A ＞ 1 and Ea/Aa ＞ 1).Transmission electron microscopy revealed a spectrum of subcellular remodeling in myocardium which was characterized by myofibril content decrease,disorganization,mitochondrial degeneration,sarcoplasmic reticulum,structural disorder.Compared with the control group,APFVF in myocardium was increased significantly in diabetic rats(P ＜ 0.05).Conclusion The diastolic dysfunction in STZ-induced diabetic rats correlates with damage of ultrastructure and increase of myocardial argyrophilic fiber.
