CAJ | 학술논문

目的观察高压氧治疗颅脑爆震伤后大鼠皮层脑组织自噬与凋亡表达的变化,探讨高压氧对脑细胞自噬和凋亡表达的影响及其意义. 方法 SD大鼠54只按照随机数字表法分为对照组(n=6)、爆震伤组(n=24)及爆震伤后高压氧治疗组(治疗组,n=24),对照组不进行致伤,爆震伤组和治疗组用600 mg TNT电雷管在大鼠头部正上方垂直距离12 cm处爆炸致伤,爆震伤组伤后不做任何治疗,治疗组分别于伤后3 h、22h及以后每天同一时间行高压氧处理,爆震伤组和治疗组分别于6 h、24 h、3 d、7 d采用RT-PCR和Western blotting检测皮层脑组织中Beclin-1和caspase-3的表达情况. 结果与对照组相比,爆震伤组及治疗组各个时间点的Beclin-1和caspase-3表达均明显增加,差异有统计学意义(P＜0.05);同一时间点上,治疗组中Beclin-1和caspase-3表达则较爆震伤组明显减少,差异有统计学意义(P＜0.05). 结论高压氧降低脑细胞的自噬和凋亡表达,此可能是其治疗颅脑损伤的机制之一.
목적 관찰고압양치료로뇌폭진상후대서피층뇌조직자서여조망표체적변화,탐토고압양대뇌세포자서화조망표체적영향급기의의. 방법 SD대서54지안조수궤수자표법분위대조조(n=6)、폭진상조(n=24)급폭진상후고압양치료조(치료조,n=24),대조조불진행치상,폭진상조화치료조용600 mg TNT전뢰관재대서두부정상방수직거리12 cm처폭작치상,폭진상조상후불주임하치료,치료조분별우상후3 h、22h급이후매천동일시간행고압양처리,폭진상조화치료조분별우6 h、24 h、3 d、7 d채용RT-PCR화Western blotting검측피층뇌조직중Beclin-1화caspase-3적표체정황. 결과 여대조조상비,폭진상조급치료조각개시간점적Beclin-1화caspase-3표체균명현증가,차이유통계학의의(P＜0.05);동일시간점상,치료조중Beclin-1화caspase-3표체칙교폭진상조명현감소,차이유통계학의의(P＜0.05). 결론 고압양강저뇌세포적자서화조망표체,차가능시기치료로뇌손상적궤제지일.
Objective To observe the changes of autophagy and apoptosis in the cerebral cortex in rats with brain explosive injury and received hyperbaric oxygen (HBO) treatment, and investigate the effect and significance of HBO on them. Methods Fifty-four SD rats were randomly divided into control group (n=6), explosive injury group (n=24) and explosive injury treated with HBO group (n=24).The control group was not injured and 600 mg TNT electric detonators were exploded over the brain of rats at a 12 cm distance in the explosive injury group and explosive injury treated with HBO group. HBO management was given to the explosive injury treated with HBO group 3 and 22 h after explosive injury and at the same time of the other 6 d. RT-PCR and Western blotting were used to detect the changes of Beclin-1 and caspase-3 in the cerebral cortex on the 6th and 24th h, 3rd and 7th d of injury. Results The expression levels of Beclin-1 and caspase-3 in the explosive injury group and explosive injury treated with HBO group were obviously higher than those in the control group (P＜0.05). The levels of Beclin-1and caspase-3 in the explosive injury treated with HBO group were significantly lower than those in the explosive injury group at the same time points (P＜0.05). Conclusion The decreased expressions of autophagy and apoptosis in brain cells by HBO treatment might be one of the possible mechanisms of treating craniocerebral injury.