CAJ | 학술논문

目的探讨针刺体壁诱导丝光绿蝇产生抗菌物质的规律. 方法室内饲养的丝光绿蝇三龄幼虫,经针刺体壁处理后,分别于第0、12、24、36、48、60、72、84 h记录幼虫活率,并提取其血淋巴,以金黄葡萄球菌作指示菌,采用平板法,作抑菌试验,37℃恒温24 h,观察抑菌圈,并以抑菌圈直径表示抗菌活力的大小,借以指示诱导丝光绿蝇抗菌物质的产生规律. 结果经针刺诱导后,丝光绿蝇幼虫活率逐渐降低,血淋巴对金黄葡萄球菌的抑菌活力峰出现在诱导后第48 h. 结论针刺体壁可诱导丝光绿蝇产生抗菌物质,免疫血淋巴收集在诱导后48 h最为合适.
목적탐토침자체벽유도사광록승산생항균물질적규률. 방법실내사양적사광록승삼령유충,경침자체벽처리후,분별우제0、12、24、36、48、60、72、84 h기록유충활솔,병제취기혈림파,이금황포도구균작지시균,채용평판법,작억균시험,37℃항온24 h,관찰억균권,병이억균권직경표시항균활력적대소,차이지시유도사광록승항균물질적산생규률. 결과경침자유도후,사광록승유충활솔축점강저,혈림파대금황포도구균적억균활력봉출현재유도후제48 h. 결론침자체벽가유도사광록승산생항균물질,면역혈림파수집재유도후48 h최위합괄.
Objective To investigate the kinetics of the inducible antibacterial activity of the hemolymph in larvae of Lucilia sericata. Methods The third instar larvae were induced by pricking treatment. Living rates of the pricked larvae were recorded at different times after treatment, and hemolymph collected at hours 0, 12, 24, 36, 48, 60, 72, 84 was tested against Staphylococcus aureus respectively, diameters of clear inhibitory ring were observed and recorded to express antibacterial activity. Results The living rates of the larvae declined gradually, and the activity peak appeared across 48 hours after treatment. Conclusion After pricking treatment, the antibacterial substances were induced in the blowfly. The immunized hemolymph should be collected at 48 hours after treatment.