CAJ | 학술논문

选择多批双黄连口服液样本,应用HPLC法测定绿原酸和连翘苷的含量,同时进行NIRS测定,建立了绿原酸和连翘苷的含量预测模型,以相对偏差(RSEP)及相关系数(r)为指标考察数据处理方法,以交互验证均方根误筹(RMSECV)为指标考察NIRS的预处理方法及选择光谱范围或波数点.数据处理方法为逐步多元线性回归(SMLR),直接采用原始光谱进行建模,绿原酸的波数为6 654.06和7 106.08 cm~(-1),连翘苷的波数为5 456.06和7 222.08 cm~(-1),最优模型预测绿原酸和连翘苷含量的RMSECV分别为0.857 26和0.889 87,相关系数分别为0.857 26和0.889 87.外部交互验证的结果表明该预测模型准确可靠,可作为双黄连口服液的快速质量评价控制方法.
선택다비쌍황련구복액양본,응용HPLC법측정록원산화련교감적함량,동시진행NIRS측정,건립료록원산화련교감적함량예측모형,이상대편차(RSEP)급상관계수(r)위지표고찰수거처리방법,이교호험증균방근오주(RMSECV)위지표고찰NIRS적예처리방법급선택광보범위혹파수점.수거처리방법위축보다원선성회귀(SMLR),직접채용원시광보진행건모,록원산적파수위6 654.06화7 106.08 cm~(-1),련교감적파수위5 456.06화7 222.08 cm~(-1),최우모형예측록원산화련교감함량적RMSECV분별위0.857 26화0.889 87,상관계수분별위0.857 26화0.889 87.외부교호험증적결과표명해예측모형준학가고,가작위쌍황련구복액적쾌속질량평개공제방법.
The aim of the present study was to establish the model of predicting the contents of chlorogenic acid and phillyrin in Shuanghuanglian oral fluid using NIR to realize quick quality evaluation of Shuanghuanglian oral fluid.To this end,many batches of Shuanghuanglian oral fluid were selected,and the contents of chlorogenic acid and phillyrin were determined using HPLC.Meanwhile,the NIR spectra of the same samples were determined.The model used to predict the contents of ehlorogenie acid and phillyrin in Shuanghuanglian oral fluid was established by correlation analysis between the results gained by HPLC and NIR spectra.According to the value of RSEP and r,the method of data processing was chosen.The method of spectra processing and wavelength range or wave numbers were chosen based on the value of RMSECV.The method of data processing was SMLR.The original spectra were used to establish the model.The wave numbers in the model used to predict the contents of chlorogenic acid and phillyrin were 6 654.06/7 106.08 cm~(-1),and 5 456.06/7 222.08 cm~(-1) respectively.The RMSECV and the correlation coefficient of the best model of chlorogenic acid and phillyrin were 0.857 26,0.889 87 and 0.857 26 and 0.889 87.The results of cross validation indicate that the predicting model was accurate and credible,and could be used as a rapid quality control method of Shuanghuanglian oral fluid.