CAJ | 학술논문

背景:伤口处细胞外基质中高含量的透明质酸对瘢痕形成有抑制作用,胚胎皮肤切口呈现无瘢痕愈合,但对于胎儿皮肤中透明质酸的含量未见报道.目的:观察胎儿颌面部皮肤中透明质酸含量及其变化趋势,并比较与其他部位的差异.方法:取32例3～8月龄引产胎儿的颌面部、背部、腹部及大腿内侧四处皮肤,按取后液氮速冻,按部位分为不同月龄组.标本称质量后剪碎,加生理盐水制成组织匀浆,取上清液,用放射免疫法测定其中透明质酸含量.结果与结论:3～8月龄胎儿颌面部皮肤中的透明质酸平均质量分数为(303.85±80.28)μ/g,腹部皮肤为(308.09±61.17)μ/g,背部皮肤为(276.38±58.52)μ/g,腿部皮肤为(304.27±60.51)μ/g.胎儿颌面部及背部皮肤中透明质酸含量3,4,5月龄高于6,7,8月龄(P<0.05),腹部皮肤4,5月龄高于6,7,8月龄(P<0.05),腿部皮肤3～6月龄高于7,8月龄(P<0.05).胎儿颌面部皮肤及其他部位皮肤透明质酸含量均高于成人;各部位皮肤均呈现孕中、后期透明质酸含量下降,但下降时间不一致.
배경:상구처세포외기질중고함량적투명질산대반흔형성유억제작용,배태피부절구정현무반흔유합,단대우태인피부중투명질산적함량미견보도.목적:관찰태인합면부피부중투명질산함량급기변화추세,병비교여기타부위적차이.방법:취32례3～8월령인산태인적합면부、배부、복부급대퇴내측사처피부,안취후액담속동,안부위분위불동월령조.표본칭질량후전쇄,가생리염수제성조직균장,취상청액,용방사면역법측정기중투명질산함량.결과여결론:3～8월령태인합면부피부중적투명질산평균질량분수위(303.85±80.28)μ/g,복부피부위(308.09±61.17)μ/g,배부피부위(276.38±58.52)μ/g,퇴부피부위(304.27±60.51)μ/g.태인합면부급배부피부중투명질산함량3,4,5월령고우6,7,8월령(P<0.05),복부피부4,5월령고우6,7,8월령(P<0.05),퇴부피부3～6월령고우7,8월령(P<0.05).태인합면부피부급기타부위피부투명질산함량균고우성인;각부위피부균정현잉중、후기투명질산함량하강,단하강시간불일치.
BACKGROUND:High level of hyaluronic acid (HA) in extracellular matrix of wounds has been shown to inhibit scar formation and scarless healing has been observed in fetal wounds.However,there Is little information regarding HA content in fetal skin.OBJECTIVE:To determine HA content in maxillofacial skin,as well as the changing tendency,and compare with other regions of skin.METHODS:The fetal skin of the maxillofacial region,back,belly,and inner part of leg were harvested from 32 aborted fetuses ranging from 3 to 8 months.All the samples were frozen immediately by liquid nitrogen and preserved at -80℃.Different month groups were designated according to skin regions.The samples were weighed and cut into pieces,then grinded into tissue homogenate.HA content in supernatant liquid of all the samples was detected using radioimmunoassay.RESULTS AND CONCLUSION:In 3-8 month-old fetuses,HA content was(303.85±80.28)μ/g in the maxillofacial skin,(308.09±61.17)μ/g in the belly skin,(276.38±58.52)μ/g in the back skin,and (304.27±60.51)μ/g in the leg skin.HA content in the maxillofacial and back skin was higher in 3-5 month-old fetuses than in 6-8 month-old fetuses (P<0.05).HA content in the leg skin was higher in the 3-6 month-old fetuses than in the 7-8 month-old fetuses (P<0.05).HA content in all parts of skin was higher in fetuses than in adults .In all parts,there was a decline of HA content in the mid-and late-stages of pregnancy,but the decline time differed.