中国医药
中國醫藥
중국의약
CHINA MEDICINE
2012年
4期
426-427
,共2页
自身免疫性溶血性贫血%直接抗人球蛋白%微柱凝胶
自身免疫性溶血性貧血%直接抗人毬蛋白%微柱凝膠
자신면역성용혈성빈혈%직접항인구단백%미주응효
Autoimmune hemolytic anemia%Direct antiglobulin%Microcolumn gel
目的 分析微柱凝胶直接抗人球蛋白试验对自身免疫性溶血性贫血诊断的效果,以便为临床提供参考.方法 选择208例交叉配血次侧阳性的标本为观察组,采用微柱凝胶直接抗人球蛋白试验；将受检红细胞分成洗涤组和非洗涤组,检测红细胞洗涤对微柱凝胶直接抗人球蛋白试验的影响；同时对此检测方法阳性结果的186例红细胞进行IgG和C3d分型.对照组(208名)采用试管法直接抗人球蛋白试验.结果 观察组与对照组阳性率比较差异有统计学意义[ 89.4％( 186/208)与53.8％ (112/208),P＜0.05].微柱凝胶直接抗人球蛋白试验生理盐水洗涤和非洗涤组受检红细胞的208份血液样本中同时检出阳性反应红细胞186份.观察组检测阳性的红细胞IgG、C3d、IgG+ C3d分型分别为46.8％ (87/186)、3.2％(6/186)、50.0％(93/186).结论 微柱凝胶直接抗人球蛋白试验对自身免疫性溶血性贫血诊断具有较高的临床应用价值.
目的 分析微柱凝膠直接抗人毬蛋白試驗對自身免疫性溶血性貧血診斷的效果,以便為臨床提供參攷.方法 選擇208例交扠配血次側暘性的標本為觀察組,採用微柱凝膠直接抗人毬蛋白試驗；將受檢紅細胞分成洗滌組和非洗滌組,檢測紅細胞洗滌對微柱凝膠直接抗人毬蛋白試驗的影響；同時對此檢測方法暘性結果的186例紅細胞進行IgG和C3d分型.對照組(208名)採用試管法直接抗人毬蛋白試驗.結果 觀察組與對照組暘性率比較差異有統計學意義[ 89.4％( 186/208)與53.8％ (112/208),P＜0.05].微柱凝膠直接抗人毬蛋白試驗生理鹽水洗滌和非洗滌組受檢紅細胞的208份血液樣本中同時檢齣暘性反應紅細胞186份.觀察組檢測暘性的紅細胞IgG、C3d、IgG+ C3d分型分彆為46.8％ (87/186)、3.2％(6/186)、50.0％(93/186).結論 微柱凝膠直接抗人毬蛋白試驗對自身免疫性溶血性貧血診斷具有較高的臨床應用價值.
목적 분석미주응효직접항인구단백시험대자신면역성용혈성빈혈진단적효과,이편위림상제공삼고.방법 선택208례교차배혈차측양성적표본위관찰조,채용미주응효직접항인구단백시험；장수검홍세포분성세조조화비세조조,검측홍세포세조대미주응효직접항인구단백시험적영향；동시대차검측방법양성결과적186례홍세포진행IgG화C3d분형.대조조(208명)채용시관법직접항인구단백시험.결과 관찰조여대조조양성솔비교차이유통계학의의[ 89.4％( 186/208)여53.8％ (112/208),P＜0.05].미주응효직접항인구단백시험생리염수세조화비세조조수검홍세포적208빈혈액양본중동시검출양성반응홍세포186빈.관찰조검측양성적홍세포IgG、C3d、IgG+ C3d분형분별위46.8％ (87/186)、3.2％(6/186)、50.0％(93/186).결론 미주응효직접항인구단백시험대자신면역성용혈성빈혈진단구유교고적림상응용개치.
Objective To analyze the effects of direct antiglobulin test with microcolumn gel in the diagnosis of autoimmune hemolytic anemia disease in order to provide references for clinic.Methods The blood specimens of 208 cases with positive blood cross-matching were selected.The tested akaryocyte were invided into ablution group and unablution group.We tested the effects of direct antiglobulin test with microcolumn gel.Meanwhile,the type of IgG and C3d was carried in 186 positive cases.In control group,the direct antiglobulin test with traditional tube was used; in the observation group there was direct antiglobulin test with microcolumn gel.Results The tested positive rate was 89.4％ in the observation group and 53.8％ in the control group; the checking method in the observation group was significantly better than that in the control group (P ＜ 0.05).Saline washed red blood cells had no effects on the results of antiglobulin test with microcolumn gel,including the same positive 186 cases.The type of IgG,C3d,IgG + C3d was 46.77％ (87/186),3.23％ (6/186),50.00％ (93/186) respectively.Conclusion The direct antiglobulin test with microcolumn gel has high clinical application value in diagnosis of autoimmune hemolytic anemia disease.
