中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2012年
5期
585-588
,共4页
八聚体转录因子类/代谢%癌,鳞状细胞/代谢/病理学%癌,基底细胞/代谢/病理学%角化病,脂溢性/代谢/病理学
八聚體轉錄因子類/代謝%癌,鱗狀細胞/代謝/病理學%癌,基底細胞/代謝/病理學%角化病,脂溢性/代謝/病理學
팔취체전록인자류/대사%암,린상세포/대사/병이학%암,기저세포/대사/병이학%각화병,지일성/대사/병이학
Octamer transcription factors/metabolism%Carcinoma,squamous cell/metabolism/pathology%Carcinoma,basal cell/metabolism/pathology%Keratosis,seborrheic/metabolism/pathology
目的 探讨表皮非黑素肿瘤中干细胞特异性标志物Oct-4的表达情况以及在表皮肿瘤发生中的作用.方法 本研究选择20例人皮肤鳞状细胞癌(SCC)、20例基底细胞癌(BCC)、20例脂溢性角化(SK)和20例正常人皮肤组织,采用EnVision免疫组化检测各组组织中干细胞特异性标志物Oct-4和增殖细胞核抗原( PCNA)的表达情况,分析其表达意义.结果 (1)Oct-4在皮肤SCC、BCC中表达强度分别为90％ (18/20)、80％(16/20),与正常皮肤组织65％ (13/20)和皮肤SK 75％(15/20)相比明显增高(P＜0.05),Oct-4在皮肤SCC和BCC之间的表达比较差异无统计学意义(P＞0.05).Oct-4在皮肤SK和正常皮肤组织之间的表达比较差异无统计学意义(P＞0.05).Oct-4在皮肤SK和BCC之间的表达比较差异无统计学意义(P＞0.05).(2)PCNA在皮肤SCC、BCC中表达强度分别为95％(19/20)、90％ (18/20),与正常皮肤组织75％ (12/20)和皮肤SK 85％(17/20)比较差异有统计学意义(P＜0.01)；在皮肤SCC和BCC之间的表达比较差异有统计学意义(P＜0.05)；在皮肤SK和正常皮肤组织之间的表达比较差异有统计学意义(P＜0.05)；而PCNA在皮肤SK和BCC的表达比较差异无统计学意义(P＞0.05).(3)Oct-4在皮肤SCC、BCC中表达强度与细胞周期蛋白PCNA表达阳性强度均呈正相关(rs=0.450,0.457,P＜0.05).结论 干细胞特异性标志物Oct-4在BCC和SCC中表达强度明显增高,可能与BCC和SCC发生发展有关.PCNA在BCC和SCC中强阳性表达,可能参与了BCC和SCC增殖过程.Oct4和PCNA在皮肤SCC、BCC中的阳性表达强度呈正相关性,Oct-4可能通过影响细胞增殖参与了BCC和SCC的发病过程.
目的 探討錶皮非黑素腫瘤中榦細胞特異性標誌物Oct-4的錶達情況以及在錶皮腫瘤髮生中的作用.方法 本研究選擇20例人皮膚鱗狀細胞癌(SCC)、20例基底細胞癌(BCC)、20例脂溢性角化(SK)和20例正常人皮膚組織,採用EnVision免疫組化檢測各組組織中榦細胞特異性標誌物Oct-4和增殖細胞覈抗原( PCNA)的錶達情況,分析其錶達意義.結果 (1)Oct-4在皮膚SCC、BCC中錶達彊度分彆為90％ (18/20)、80％(16/20),與正常皮膚組織65％ (13/20)和皮膚SK 75％(15/20)相比明顯增高(P＜0.05),Oct-4在皮膚SCC和BCC之間的錶達比較差異無統計學意義(P＞0.05).Oct-4在皮膚SK和正常皮膚組織之間的錶達比較差異無統計學意義(P＞0.05).Oct-4在皮膚SK和BCC之間的錶達比較差異無統計學意義(P＞0.05).(2)PCNA在皮膚SCC、BCC中錶達彊度分彆為95％(19/20)、90％ (18/20),與正常皮膚組織75％ (12/20)和皮膚SK 85％(17/20)比較差異有統計學意義(P＜0.01)；在皮膚SCC和BCC之間的錶達比較差異有統計學意義(P＜0.05)；在皮膚SK和正常皮膚組織之間的錶達比較差異有統計學意義(P＜0.05)；而PCNA在皮膚SK和BCC的錶達比較差異無統計學意義(P＞0.05).(3)Oct-4在皮膚SCC、BCC中錶達彊度與細胞週期蛋白PCNA錶達暘性彊度均呈正相關(rs=0.450,0.457,P＜0.05).結論 榦細胞特異性標誌物Oct-4在BCC和SCC中錶達彊度明顯增高,可能與BCC和SCC髮生髮展有關.PCNA在BCC和SCC中彊暘性錶達,可能參與瞭BCC和SCC增殖過程.Oct4和PCNA在皮膚SCC、BCC中的暘性錶達彊度呈正相關性,Oct-4可能通過影響細胞增殖參與瞭BCC和SCC的髮病過程.
목적 탐토표피비흑소종류중간세포특이성표지물Oct-4적표체정황이급재표피종류발생중적작용.방법 본연구선택20례인피부린상세포암(SCC)、20례기저세포암(BCC)、20례지일성각화(SK)화20례정상인피부조직,채용EnVision면역조화검측각조조직중간세포특이성표지물Oct-4화증식세포핵항원( PCNA)적표체정황,분석기표체의의.결과 (1)Oct-4재피부SCC、BCC중표체강도분별위90％ (18/20)、80％(16/20),여정상피부조직65％ (13/20)화피부SK 75％(15/20)상비명현증고(P＜0.05),Oct-4재피부SCC화BCC지간적표체비교차이무통계학의의(P＞0.05).Oct-4재피부SK화정상피부조직지간적표체비교차이무통계학의의(P＞0.05).Oct-4재피부SK화BCC지간적표체비교차이무통계학의의(P＞0.05).(2)PCNA재피부SCC、BCC중표체강도분별위95％(19/20)、90％ (18/20),여정상피부조직75％ (12/20)화피부SK 85％(17/20)비교차이유통계학의의(P＜0.01)；재피부SCC화BCC지간적표체비교차이유통계학의의(P＜0.05)；재피부SK화정상피부조직지간적표체비교차이유통계학의의(P＜0.05)；이PCNA재피부SK화BCC적표체비교차이무통계학의의(P＞0.05).(3)Oct-4재피부SCC、BCC중표체강도여세포주기단백PCNA표체양성강도균정정상관(rs=0.450,0.457,P＜0.05).결론 간세포특이성표지물Oct-4재BCC화SCC중표체강도명현증고,가능여BCC화SCC발생발전유관.PCNA재BCC화SCC중강양성표체,가능삼여료BCC화SCC증식과정.Oct4화PCNA재피부SCC、BCC중적양성표체강도정정상관성,Oct-4가능통과영향세포증식삼여료BCC화SCC적발병과정.
Objective The aim of this study was to understand the role of specific markers of stem cells Oct-4 expression in the development of human epidermal non-melanoma cutaneous tumors.Methods The paraffin-embedded samples were retrieved from files of pathology department at our hospital,including 20 cases of skin squamous cell carcinomas (SCC),20 cases of basal cell carcinomas (BCC),20 cases of seborrhoeic keratosis (SK) and 20 cases of normal skin (from head,face,trunk,extremities).The expression of Oct-4 and PCNA were observed by immunohistochemical staining technique.Results Oct-4 protein was abnormally increased in SCC and BCC comparel to normal skin and SK ( P ＜0.05 ).However there were no significant difference of Oct-4 protein expression between SCC and BCC ( P ＞0.05).There were also no significantly different Oct-4 protein expression between Sk and the normal skin ( P ＞ 0.05 ),and no significantly different Oct-4 protein expression between SK and BCC( P ＞0.05 ).PCNA protein was abnormally increased in SCC and BCC compared to normal skin and SK ( P ＜0.01 ).There were significantly different PCNA protein expression between SCC and BCC( P ＜0.05).There were also significantly different PCNA protein expression between SK and the normal skin ( P ＜ 0.05 ).However there were no significant difference of PCNA protein expression between SK and BCC ( P ＞ 0.05 ).There were positive correlation between the expression intensity of Oct-4 and PCNA in SCC and BCC.Conclusions The abnormal expression of Oct-4 may have an important role in the development of BCC and SCC.Positive Oct-4 expression cells may be the tumor stem cell in SCC and BCC.There were positive correlation between the expression intensity of Oct-4 and PCNA in SCC and BCC.The over expression Oct-4 in BCC and SCC may play an important role in proliferation of tumor.
