中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2011年
11期
846-848
,共3页
血管内皮细胞%放射性损伤%凋亡%细胞内活性氧
血管內皮細胞%放射性損傷%凋亡%細胞內活性氧
혈관내피세포%방사성손상%조망%세포내활성양
Vascular endothelial cells%Radiation damage%Apoptosis%Intracellular reactive oxygen species
目的 探讨姜黄素对放射性血管内皮损伤的保护作用及其机制.方法 对人脐静脉内皮细胞(HUVEC)分离培养后分组,以2、4、6和8Gy4种不同剂量进行β线照射,并设未照射组为对照.分别通过光学显微镜及电子显微镜观察细胞形态和超微结构改变；流式细胞仪法观察各组的凋亡率和坏死率以及细胞内活性氧(ROS)生成；检测细胞照射前后乳酸脱氢酶(LDH)活力和丙二醛(MDA)含量的变化.结果 细胞经照射后,形态及超微结构改变均显示为典型的凋亡改变,而姜黄素预处理组的细胞凋亡率和坏死率均明显轻于其他实验组,差别有统计学意义(P＜0.05).LDH活力和MDA含量在各照射组均高于姜黄素预处理组(P＜0.05),照射后各组ROS含量明显升高,呈剂量依赖性改变,明显高于姜黄素预处理组,差别均有统计学意义(P＜0.05).结论 放射线照射可导致HUVEC凋亡和坏死,细胞内LDH活力和MDA含量增高,且呈剂量依赖性.姜黄素对受照射后的HUVEC凋亡和坏死有保护作用.
目的 探討薑黃素對放射性血管內皮損傷的保護作用及其機製.方法 對人臍靜脈內皮細胞(HUVEC)分離培養後分組,以2、4、6和8Gy4種不同劑量進行β線照射,併設未照射組為對照.分彆通過光學顯微鏡及電子顯微鏡觀察細胞形態和超微結構改變；流式細胞儀法觀察各組的凋亡率和壞死率以及細胞內活性氧(ROS)生成；檢測細胞照射前後乳痠脫氫酶(LDH)活力和丙二醛(MDA)含量的變化.結果 細胞經照射後,形態及超微結構改變均顯示為典型的凋亡改變,而薑黃素預處理組的細胞凋亡率和壞死率均明顯輕于其他實驗組,差彆有統計學意義(P＜0.05).LDH活力和MDA含量在各照射組均高于薑黃素預處理組(P＜0.05),照射後各組ROS含量明顯升高,呈劑量依賴性改變,明顯高于薑黃素預處理組,差彆均有統計學意義(P＜0.05).結論 放射線照射可導緻HUVEC凋亡和壞死,細胞內LDH活力和MDA含量增高,且呈劑量依賴性.薑黃素對受照射後的HUVEC凋亡和壞死有保護作用.
목적 탐토강황소대방사성혈관내피손상적보호작용급기궤제.방법 대인제정맥내피세포(HUVEC)분리배양후분조,이2、4、6화8Gy4충불동제량진행β선조사,병설미조사조위대조.분별통과광학현미경급전자현미경관찰세포형태화초미결구개변；류식세포의법관찰각조적조망솔화배사솔이급세포내활성양(ROS)생성；검측세포조사전후유산탈경매(LDH)활력화병이철(MDA)함량적변화.결과 세포경조사후,형태급초미결구개변균현시위전형적조망개변,이강황소예처리조적세포조망솔화배사솔균명현경우기타실험조,차별유통계학의의(P＜0.05).LDH활력화MDA함량재각조사조균고우강황소예처리조(P＜0.05),조사후각조ROS함량명현승고,정제량의뢰성개변,명현고우강황소예처리조,차별균유통계학의의(P＜0.05).결론 방사선조사가도치HUVEC조망화배사,세포내LDH활력화MDA함량증고,차정제량의뢰성.강황소대수조사후적HUVEC조망화배사유보호작용.
Objective To study the effects of curcumin on vascular endothelial injuries induced by radiation and the mechanism.Methods Human umbilical vein endothelial cells (HUVEC) were isolated,cultured and divided into the control group and 4 groups exposed to 3-ray at the doses of 2,4,6 and 8 Gy.Cellular morphological and ultrastructural changes were examined under light microscopy and electron microscopy respectively.Flow cytometry was used to detect the cellular apoptosis,necrosis and intracellular reactive oxygen species (ROS) generation.The contents of Lactate dehydrogenase (LDH) and malondialdehyde (MDA) in the cultures were measured before and after irradiation.Results The results of cellular morphological and ultrastructural examinations shown that the typical apoptotic changes appeared after irradiation.The rates of apoptosis and necrosis in groups pretreated with curcumin were significantly lower than those in other groups (P＜0.05).LDH and MDA in the irradiation groups were significantly higher than those in curcumin pretreatment groups (P＜0.05).The ROS generation in radiation groups significantly increased with the radiation doses,as compared with the groups pretreated with curcumin (P＜0.05).Conclusion Irradiation induced the apoptosis and necrosis of HUVEC,and increased significantly the intracellular LDH and MDA levels in a dose-dependent.Curcumin had the protective effects on HUVEC from the apoptosis and necrosis induced by radiation.
