CAJ | 학술논문

目的探讨原发性干燥综合征(pSS)患者外周血B细胞FcγRⅡb的表达及其临床意义.方法流式细胞术检测19例pSS患者、15名健康对照外周血B细胞FcγRⅡb的平均荧光强度(MFI)；酶联免疫吸附试验( ELISA)方法测定19例pSS患者血清抗SSA、SSB抗体水平,统计学分析采用t检验、单因素方差分析、SNK-q检验及Pearson相关分析.结果 pSS患者CD 19+CD27+记忆性B细胞亚群的百分率[(20.8±2.7)％,19例]显著低于健康对照组[(37.8±2.2)％,15名](t=-4.002,P＜0.01)；活动期pSS患者外周血CD19+CD27+记忆性B细胞FcγRⅡb的MFI[( 74±8),13例]低于非活动期组[(132±11),6例]及健康对照组[(139±12),15名](F=10.699,P＜0.01)；pSS患者外周血CD19+CD27+记忆性B细胞FcγRⅡb的MFI与pSS疾病活动指数(SSDAI)呈负相关(r=-0.744,P=0.0003)；抗SSA、SSB抗体阳性组pSS患者CD19+CD27+记忆性B细胞FcγRⅡb的MFI[分别为(75±3),12例；(48±7),5例]显著低于抗SSA、SSB抗体阴性组[分别为(122±11),7例；(108±9),14例](t分别为-4.336和-3.776,P均＜0.01)；抗SSA抗体阳性组pSS患者CD19+CD27+记忆性B细胞FcγRⅡb的MFI与抗SSA抗体滴度呈负相关(r=-0.685,P=0.014).结论 pSS患者活动期外周血记忆性B细胞FcyRⅡb表达与SSDAI呈负相关,并与抗SSA抗体呈负相关.FcγR Ⅱb表达异常可能在pSS免疫发病机制中起重要作用.
목적 탐토원발성간조종합정(pSS)환자외주혈B세포FcγRⅡb적표체급기림상의의.방법 류식세포술검측19례pSS환자、15명건강대조외주혈B세포FcγRⅡb적평균형광강도(MFI)；매련면역흡부시험( ELISA)방법측정19례pSS환자혈청항SSA、SSB항체수평,통계학분석채용t검험、단인소방차분석、SNK-q검험급Pearson상관분석.결과 pSS환자CD 19+CD27+기억성B세포아군적백분솔[(20.8±2.7)％,19례]현저저우건강대조조[(37.8±2.2)％,15명](t=-4.002,P＜0.01)；활동기pSS환자외주혈CD19+CD27+기억성B세포FcγRⅡb적MFI[( 74±8),13례]저우비활동기조[(132±11),6례]급건강대조조[(139±12),15명](F=10.699,P＜0.01)；pSS환자외주혈CD19+CD27+기억성B세포FcγRⅡb적MFI여pSS질병활동지수(SSDAI)정부상관(r=-0.744,P=0.0003)；항SSA、SSB항체양성조pSS환자CD19+CD27+기억성B세포FcγRⅡb적MFI[분별위(75±3),12례；(48±7),5례]현저저우항SSA、SSB항체음성조[분별위(122±11),7례；(108±9),14례](t분별위-4.336화-3.776,P균＜0.01)；항SSA항체양성조pSS환자CD19+CD27+기억성B세포FcγRⅡb적MFI여항SSA항체적도정부상관(r=-0.685,P=0.014).결론 pSS환자활동기외주혈기억성B세포FcyRⅡb표체여SSDAI정부상관,병여항SSA항체정부상관.FcγR Ⅱb표체이상가능재pSS면역발병궤제중기중요작용.
Objective To investigate the expression of FcγRⅡb on peripheral B cells and its clinical significance in primary Sj(o)gren's syndrome (pSS).Methods FcγRⅡb expression on peripheral B cells from 19 pSS patients and 15 healthy controls was examined by flow cytometry.The levels of serum anti-SSA and SSB antibodies were determined using enzyme-linked immunosorbent assay (ELISA).Data were analyzed with t test,one-way ANOVA,SNK-q test and Pearson's correlation test.Results The percentage of memory CD19+CD27+ B cell subpopulation was significantly lower in pSS patients [ (20.8±2.7)％] when compared to normal controls [(37.8±2.2)％ ](t=-4.002,P＜0.01).The level of expression of FcγRⅡb in active pSS memory CD19+CD27+ B cells [ MFI(74±8)] was significantly reduced when compared to inactive [ MFI( 132±11)] and normal controls [ MFI (139±12)] (F=10.699,P＜0.01).The level of expression of FcγRⅡb on memory CD19+CD27+ B cells from pSS patients was inversely correlated with Sj(o)gren's syndrome disease activity index (SSDAI) (r=-0.744,P=0.0003 ).pSS patients with the serum anti-SSA/SSB antibodies positive group [ MFI(75+3),(48±7)] displayed a lower expression of FcγRⅡb on memory CD19+CD27+ B cell than in patients with the serum anti-SSA/SSB antibodies negative group [MFI( 122±11),(108±9)] (t=-4.336 and -3.776 respectively,the P value of both tests were less than 0.01).The level of expression of FcγRⅡb in the memory CD19+CD27+ B cells of patients with active pSS was inversely correlated with anti-SSA antibody titers (r=-0.685,P=0.014).Conclusion The expression of FcγRⅡb on peripheral memory B cells from active pSS patients is inversely correlated with SSDAI and is also inversely associated with anti-SSA antibody levels.Decreased expression of FcγRⅡb might play an important role in the pathogenesis of pSS.