中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2009年
2期
135-137
,共3页
肝炎,乙型%肝炎e抗原,乙型%病毒包膜蛋白质类%肝炎病毒,乙型%DNA
肝炎,乙型%肝炎e抗原,乙型%病毒包膜蛋白質類%肝炎病毒,乙型%DNA
간염,을형%간염e항원,을형%병독포막단백질류%간염병독,을형%DNA
Hepatitis B%Hepatitis B e antigens%Viral envelope protein%Hepatitis B virus%DNA
目的 探讨血清乙型肝炎病毒外膜大蛋白(HBV-LP)检测在HBeAg阴性慢性乙型肝炎中的诊断价值和临床意义.方法 分别采用酶联免疫吸附试验(ELISA)法和实时荧光定量PCR法检测306份慢性乙型肝炎患者血清中HBV.M、HBV-LP和HBV.DNA.结果 (1)在未经过抗病毒治疗的患者中,HBV-LP阳性率和HBV DNA无明显差异,而且HBV-LP的吸光度(A值)和HBV DNA拷贝数呈正相关性;(2)在经过抗病毒治疗的患者中HBV-LP的阳性率明显高于HBV DNA.结论 血清中乙型肝炎病毒大蛋白水平能反映HBV感染者体内HBV复制程度,对于判断HBeAg阴性患者体内复制具有重要的临床意义.
目的 探討血清乙型肝炎病毒外膜大蛋白(HBV-LP)檢測在HBeAg陰性慢性乙型肝炎中的診斷價值和臨床意義.方法 分彆採用酶聯免疫吸附試驗(ELISA)法和實時熒光定量PCR法檢測306份慢性乙型肝炎患者血清中HBV.M、HBV-LP和HBV.DNA.結果 (1)在未經過抗病毒治療的患者中,HBV-LP暘性率和HBV DNA無明顯差異,而且HBV-LP的吸光度(A值)和HBV DNA拷貝數呈正相關性;(2)在經過抗病毒治療的患者中HBV-LP的暘性率明顯高于HBV DNA.結論 血清中乙型肝炎病毒大蛋白水平能反映HBV感染者體內HBV複製程度,對于判斷HBeAg陰性患者體內複製具有重要的臨床意義.
목적 탐토혈청을형간염병독외막대단백(HBV-LP)검측재HBeAg음성만성을형간염중적진단개치화림상의의.방법 분별채용매련면역흡부시험(ELISA)법화실시형광정량PCR법검측306빈만성을형간염환자혈청중HBV.M、HBV-LP화HBV.DNA.결과 (1)재미경과항병독치료적환자중,HBV-LP양성솔화HBV DNA무명현차이,이차HBV-LP적흡광도(A치)화HBV DNA고패수정정상관성;(2)재경과항병독치료적환자중HBV-LP적양성솔명현고우HBV DNA.결론 혈청중을형간염병독대단백수평능반영HBV감염자체내HBV복제정도,대우판단HBeAg음성환자체내복제구유중요적림상의의.
Objective To explore the clinical significance of hepatitis B virus large envelope protein (HBV-LP) in diagnosing viral replication,we detected the HBV-LP and HBV DNA in the serttrn of the patients infected with HBV. Methods Serum HBV DNA was detected by fluorescent quantitative PCR (FQ-PCR). HBV- LP was analyzed by enzyme linked immunosorbent assay( ELISA). Results (1) No significant difference between the detectable rate of HBV DNA and HBV-LP was found in the HBeAg negative patients without of anti-virus treatment. Of all HBeAg negative patients without of anti-virus treatment, the copies of HBV-DNA was related positively to the absorbency HBV-LP. (2) There was significant diference between the detectable rate of HBV DNA and HBV-LP in the HBeAg negative patients after anti-virus treatment.Conclusion There is a perfect correlation between the vositive rate of HBV-LP and HBV DNA in the HBeAg negative patients. The HBV DNA is a reliable serological marker that can reflect the replication of HBV in the HBeAg negative patients.
