中华显微外科杂志
中華顯微外科雜誌
중화현미외과잡지
Chinese Journal of Microsurgery
2010年
1期
38-40
,共3页
黄永军%黄东%牟勇%吴伟炽%张惠茹%葛军委%江钦文
黃永軍%黃東%牟勇%吳偉熾%張惠茹%葛軍委%江欽文
황영군%황동%모용%오위치%장혜여%갈군위%강흠문
碱性成纤维细胞生长因子%新生血管化%皮瓣%移植
堿性成纖維細胞生長因子%新生血管化%皮瓣%移植
감성성섬유세포생장인자%신생혈관화%피판%이식
Basic fibroblast growth factor%Neovascularization%Flap%Transplantation
目的 探讨碱性成纤维细胞生长因子(bFGF)对大鼠移植皮瓣成活作用的影响.方法 Wistar大鼠48只,按月龄随机分为A、B、C、D四组,每组12只.制作移植皮瓣模型.A、C组的大鼠多次多点每点注射bFGF 60 U(0.015 ml);B、D组给予等量生理盐水作为对照.分别于5 d、14 d观察皮瓣的存活率,分别取相同部位皮瓣组织块行病理组织学检查,并采用计算机图像分析系统和免疫组化方法计算血管密度和血管内皮生长因子(VEGF)阳性血管数.结果 大鼠缺血皮瓣血管密度及VEGF阳性表达的血管数A组[(分别为(134.21±4.86)个/mm~2,(14.63±2.25)条]多于B组[分别为(118.48±2.31)个/mm~2,(7.45±1.43)条],C组[分别为(128.67±4.58)个/mm~2,(11.39±1.61)条]多于D组[分别为(115.32±2.18)个/mm~2,(6.96±1.35)条],差异均有统计学意义(P<0.05);A组和C组比较,差异无统计学意义(P>0.05).结论 在不同月龄大鼠移植皮瓣内注射bFGF蛋白可促进皮瓣血管新生,改善移植皮瓣缺血状态,提高皮瓣成活率.
目的 探討堿性成纖維細胞生長因子(bFGF)對大鼠移植皮瓣成活作用的影響.方法 Wistar大鼠48隻,按月齡隨機分為A、B、C、D四組,每組12隻.製作移植皮瓣模型.A、C組的大鼠多次多點每點註射bFGF 60 U(0.015 ml);B、D組給予等量生理鹽水作為對照.分彆于5 d、14 d觀察皮瓣的存活率,分彆取相同部位皮瓣組織塊行病理組織學檢查,併採用計算機圖像分析繫統和免疫組化方法計算血管密度和血管內皮生長因子(VEGF)暘性血管數.結果 大鼠缺血皮瓣血管密度及VEGF暘性錶達的血管數A組[(分彆為(134.21±4.86)箇/mm~2,(14.63±2.25)條]多于B組[分彆為(118.48±2.31)箇/mm~2,(7.45±1.43)條],C組[分彆為(128.67±4.58)箇/mm~2,(11.39±1.61)條]多于D組[分彆為(115.32±2.18)箇/mm~2,(6.96±1.35)條],差異均有統計學意義(P<0.05);A組和C組比較,差異無統計學意義(P>0.05).結論 在不同月齡大鼠移植皮瓣內註射bFGF蛋白可促進皮瓣血管新生,改善移植皮瓣缺血狀態,提高皮瓣成活率.
목적 탐토감성성섬유세포생장인자(bFGF)대대서이식피판성활작용적영향.방법 Wistar대서48지,안월령수궤분위A、B、C、D사조,매조12지.제작이식피판모형.A、C조적대서다차다점매점주사bFGF 60 U(0.015 ml);B、D조급여등량생리염수작위대조.분별우5 d、14 d관찰피판적존활솔,분별취상동부위피판조직괴행병리조직학검사,병채용계산궤도상분석계통화면역조화방법계산혈관밀도화혈관내피생장인자(VEGF)양성혈관수.결과 대서결혈피판혈관밀도급VEGF양성표체적혈관수A조[(분별위(134.21±4.86)개/mm~2,(14.63±2.25)조]다우B조[분별위(118.48±2.31)개/mm~2,(7.45±1.43)조],C조[분별위(128.67±4.58)개/mm~2,(11.39±1.61)조]다우D조[분별위(115.32±2.18)개/mm~2,(6.96±1.35)조],차이균유통계학의의(P<0.05);A조화C조비교,차이무통계학의의(P>0.05).결론 재불동월령대서이식피판내주사bFGF단백가촉진피판혈관신생,개선이식피판결혈상태,제고피판성활솔.
Objective To investigate the application valve of bFGF to improve the viability of trans-plantation flap in Wistar rat. Methods Forty-eight Wistar rats were randomly divided into two groups based on the age: group A,B(1.5 month old, every group was 12 rats) and group C,D (3 month old, every group was 12 rats). After an ischemic model completed, recombinant bovine basic fibroblast growth factor (rb-bFGF) was given to groups A and C in ischemie zone by vascularization flap injection and the equality of normal saline to groups B and D. 14 days postoperatively, the muscular tissue was sent for histology, the blood vessel density was calculated by image analysis, and positive VEGF was detected by immunohistochem-istry. Results The member of capillaries and positive VEGF were more in group A than that in B, and also were morein group C than D(P < 0.05), but there were not statistic difference between group A and C (P > 0.05). Conclusion Recombinant bovine bFGF can stimulate angiogenesis and improve the ischemie vascu-larization flap of rat, which is not associated with their age.
