中华血液学杂志
中華血液學雜誌
중화혈액학잡지
Chinese Journal of Hematology
2012年
7期
536-540
,共5页
程翼飞%张乐萍%刘艳荣%秦亚溱%吴珺%刘桂兰
程翼飛%張樂萍%劉豔榮%秦亞溱%吳珺%劉桂蘭
정익비%장악평%류염영%진아진%오군%류계란
基因,WT1%基因表达%骨髓增生异常综合征%儿童
基因,WT1%基因錶達%骨髓增生異常綜閤徵%兒童
기인,WT1%기인표체%골수증생이상종합정%인동
Gene,Wilms' tumor 1%Gene expression%Myelodysplastic syndrome%Children
目的 探讨儿童骨髓增生异常综合征(MDS)中WT1基因表达规律及其在鉴别MDS与再生障碍性贫血(AA)中的作用.方法 采集2008年9月至2011年12月收治的36例儿童MDS、49例儿童AA及40例儿童急性髓系白血病(AML)患者骨髓细胞,采用实时荧光定量PCR法分析其WT1表达规律.结果 ①重型AA组患儿WT1基因表达阳性率为0,慢性AA组为14.3%,MDS-难治性血细胞减少(RC)组为58.6%,难治性贫血伴原始细胞增多(RAEB)组为100%,AML组为97.5%.MDS-RC组WT1基因表达阳性率高于重型AA组和慢性AA组(P值分别为0.000和0.001).重型AA、慢性AA、MDS-RC、MDS-RAEB、AML组WT1基因相对表达水平平均值分别为0.041%、0.357%、7.037%、12.680%、24.210%.②低增生MDS组WT1基因表达阳性率为66.7%,高于慢性AA组(P=0.001)和重型AA组(P=0.000);低增生MDS患儿WT1相对表达水平为(3.022±5.040)%,高于慢性AA组[(0.357±0.479)%,P=0.002]及重型AA组[(0.041±0.047)%,P=0.000].结论 MDS患儿WT1基因表达水平较AA为高;WT1基因表达水平随着MDS疾病的进展而增高; WT1水平可用于鉴别儿童低增生MDS与AA.
目的 探討兒童骨髓增生異常綜閤徵(MDS)中WT1基因錶達規律及其在鑒彆MDS與再生障礙性貧血(AA)中的作用.方法 採集2008年9月至2011年12月收治的36例兒童MDS、49例兒童AA及40例兒童急性髓繫白血病(AML)患者骨髓細胞,採用實時熒光定量PCR法分析其WT1錶達規律.結果 ①重型AA組患兒WT1基因錶達暘性率為0,慢性AA組為14.3%,MDS-難治性血細胞減少(RC)組為58.6%,難治性貧血伴原始細胞增多(RAEB)組為100%,AML組為97.5%.MDS-RC組WT1基因錶達暘性率高于重型AA組和慢性AA組(P值分彆為0.000和0.001).重型AA、慢性AA、MDS-RC、MDS-RAEB、AML組WT1基因相對錶達水平平均值分彆為0.041%、0.357%、7.037%、12.680%、24.210%.②低增生MDS組WT1基因錶達暘性率為66.7%,高于慢性AA組(P=0.001)和重型AA組(P=0.000);低增生MDS患兒WT1相對錶達水平為(3.022±5.040)%,高于慢性AA組[(0.357±0.479)%,P=0.002]及重型AA組[(0.041±0.047)%,P=0.000].結論 MDS患兒WT1基因錶達水平較AA為高;WT1基因錶達水平隨著MDS疾病的進展而增高; WT1水平可用于鑒彆兒童低增生MDS與AA.
목적 탐토인동골수증생이상종합정(MDS)중WT1기인표체규률급기재감별MDS여재생장애성빈혈(AA)중적작용.방법 채집2008년9월지2011년12월수치적36례인동MDS、49례인동AA급40례인동급성수계백혈병(AML)환자골수세포,채용실시형광정량PCR법분석기WT1표체규률.결과 ①중형AA조환인WT1기인표체양성솔위0,만성AA조위14.3%,MDS-난치성혈세포감소(RC)조위58.6%,난치성빈혈반원시세포증다(RAEB)조위100%,AML조위97.5%.MDS-RC조WT1기인표체양성솔고우중형AA조화만성AA조(P치분별위0.000화0.001).중형AA、만성AA、MDS-RC、MDS-RAEB、AML조WT1기인상대표체수평평균치분별위0.041%、0.357%、7.037%、12.680%、24.210%.②저증생MDS조WT1기인표체양성솔위66.7%,고우만성AA조(P=0.001)화중형AA조(P=0.000);저증생MDS환인WT1상대표체수평위(3.022±5.040)%,고우만성AA조[(0.357±0.479)%,P=0.002]급중형AA조[(0.041±0.047)%,P=0.000].결론 MDS환인WT1기인표체수평교AA위고;WT1기인표체수평수착MDS질병적진전이증고; WT1수평가용우감별인동저증생MDS여AA.
Objective To investigate the expression of the Wilms' tumor
1(WT1) mRNA in childhood myelodysplastic syndrome(MDS), and to evaluate WT1 as a tool to differentiate MDS from aplastic anemia(AA).Methods The quantitative expression of WT1 transcript by using real-time quantitative polymerase chain reaction(RQ-PCR) was performed in the bone marrow samples of 36 childhood MDS and 49 childhood AA, the samples were collected from September 2008 to December 2011.Results ①The positive rate of WT1 in severe AA(SAA) was 0, 14.3% in chronic AA(CAA), 58.6% in refractory cytopenia(RC), 100% in refractory anemia with
excessive blast(RAEB) and 97.5% in acute myeloid leukemia(AML).The mean level of WT1 in SAA, CAA, RC, RAEB and AML was 0.041%, 0.357%, 7.037%, 12.680% and 24.210%, respectivly.The positive rate of WT1 in RC patients was higher than that of SAA(P=0.000)and CAA(P=0.001).②The positive rate of WT1 in patients with hypoplastic MDS was 66.7% and was higher than that of SAA(P=0.000)and CAA(P=0.001).The mean level of WT1 in patients with hypoplastic MDS was (3.022±5.040)% and higher than that of SAA [(0.041±0.047)%,P=0.000] and [(0.351±0.479)%, P=0.002]. Conclusions The level of WT1 in childhood MDS was higher than that of childhood AA. The degree of WT1 expression in MDS increased during disease progression. WT1 is a useful tool for differentiating the childhood hypoplastic MDS from AA.
