中国实用医刊
中國實用醫刊
중국실용의간
CENTRAL PLAINS MEDICAL JOURNAL
2010年
7期
29-31
,共3页
形觉剥夺性近视眼%褪黑素%诱导型一氧化氮合酶
形覺剝奪性近視眼%褪黑素%誘導型一氧化氮閤酶
형각박탈성근시안%퇴흑소%유도형일양화담합매
FDM%Melatonini%NOS
目的 建立豚鼠形觉剥夺性近视眼(FDM)动物模型,观察视网膜中褪黑素和诱导型一氧化氮合酶(iNOS)在FDM中的变化及作用.方法 将出生7 d的30只豚鼠用半透明眼罩遮盖右眼,左眼作为对照,遮盖8周,然后检影验光检测双眼屈光度,A超测量双眼眼轴长度;免疫组化法分析褪黑素和iNOS的表达.结果 形觉剥夺使眼轴增长,近视屈光度增加,视网膜中褪黑素免疫活性降低而iNOS的活性增加(P＜0.05).结论 褪黑素和iNOS可能参与调控FDM的形成,褪黑素和iNOS之间呈负相关,也可能同时受其他因子的调控.
目的 建立豚鼠形覺剝奪性近視眼(FDM)動物模型,觀察視網膜中褪黑素和誘導型一氧化氮閤酶(iNOS)在FDM中的變化及作用.方法 將齣生7 d的30隻豚鼠用半透明眼罩遮蓋右眼,左眼作為對照,遮蓋8週,然後檢影驗光檢測雙眼屈光度,A超測量雙眼眼軸長度;免疫組化法分析褪黑素和iNOS的錶達.結果 形覺剝奪使眼軸增長,近視屈光度增加,視網膜中褪黑素免疫活性降低而iNOS的活性增加(P＜0.05).結論 褪黑素和iNOS可能參與調控FDM的形成,褪黑素和iNOS之間呈負相關,也可能同時受其他因子的調控.
목적 건립돈서형각박탈성근시안(FDM)동물모형,관찰시망막중퇴흑소화유도형일양화담합매(iNOS)재FDM중적변화급작용.방법 장출생7 d적30지돈서용반투명안조차개우안,좌안작위대조,차개8주,연후검영험광검측쌍안굴광도,A초측량쌍안안축장도;면역조화법분석퇴흑소화iNOS적표체.결과 형각박탈사안축증장,근시굴광도증가,시망막중퇴흑소면역활성강저이iNOS적활성증가(P＜0.05).결론 퇴흑소화iNOS가능삼여조공FDM적형성,퇴흑소화iNOS지간정부상관,야가능동시수기타인자적조공.
Objective Using a guinea pig model to explore the effects of retinal melatonin and iNOS on FDM.Methods Thirty guineas 7 day - old, their right eyes were worn with monocular translucent goggles for 8 weeks, the left eyes were contral eyes.The axial length was measured with A - mode ultrasound, and retino -scopic refraction was performed.Melatonin and iNOS was analysed with immunohistochemistry.Results The axial length of occluded eyes were significantly longer than control eyes (P ＜ 0.05).Form -deprivation eyes were significantly more myopia than control eyes(P ＜ 0.05).Form - deprivation reduced the immunostaining of melatonin, increased the immunostaining of iNOS.Conclusions Melatonin and iNOS may be involved in the positive regulation in FDM.The similar expression of melatonin and iNOS implied some interaction between the melatonin and iNOS, probably other factors also involve in the regulation procedure in FDM.
