中华检验医学杂志
中華檢驗醫學雜誌
중화검험의학잡지
CHINESE JOURNAL OF LABORATORY MEDICINE
2010年
12期
1133-1138
,共6页
宁乐平%刘松梅%周新%郑芳%徐凤霞
寧樂平%劉鬆梅%週新%鄭芳%徐鳳霞
저악평%류송매%주신%정방%서봉하
糖尿病,2型%脂肪酸类,非酯化%高脂血症%色谱法,高压液相
糖尿病,2型%脂肪痠類,非酯化%高脂血癥%色譜法,高壓液相
당뇨병,2형%지방산류,비지화%고지혈증%색보법,고압액상
Diabetes mellitus,type 2%Fatty acids,nonesterified%Hyperlipidemias%Chromatography,high pressure liquid
目的 用高效液相色谱法分析T2DM患者血浆游离脂肪酸谱的变化.方法 收集武汉大学中南医院体检人群和住院患者,其中健康对照组94名、单纯T2DM组101例、T2DM合并高脂血症组77例.取受试者空腹静脉血,分离新鲜血浆,以正十七酸作为内标,以改良Doles法萃取游离脂肪酸,溴苯乙酮作为柱前衍化剂,采用Dionex Summit高效液相色谱法分析系统检测血浆游离脂肪酸谱,内标标准曲线法定量.结果 健康对照组、单纯T2DM组与T2DM合并高脂血症组3组之间的总游离脂肪酸浓度分别为(355.63±100.35)、(421.21±200.83)、(473.04±213.40)μmol/L,3组之间差异有统计学意义(X2=13.08,P<0.01),不饱和游离脂肪酸浓度依次为(206.29±61.94)、(218.11±110.28)、(240.94±116.79)μmol/L,3组之间差异无统计学意义(x2=2.17,P>0.05).单纯T2DM组与健康对照组比较,总游离脂肪酸升高[(421.21±200.83)μmol/L vs(355.63±100.35)μmol/L,x2=7.22,P<0.05],不饱和游离脂肪酸浓度和健康对照组差异无统计学意义.T2DM合并高脂血症组与健康对照组比较,总游离脂肪酸浓度升高[(473.04±213.40)μmol/L vs (355.63±100.35)μmol/L,x2=27.93,P<0.01].日间及日内RSD均<5%,最低检测浓度为0.05～0.35 μmol/L,高、中、低浓度下的回收率为96.4%～104.8%.结论 T2DM患者血浆总游离脂肪酸浓度升高,以饱和游离脂肪酸升高为主,不饱和游离脂肪酸改变差异无统计学意义.可能与T2MD的发生发展有关,有望成为糖尿病脂代谢紊乱的临床早期监测新指标.
目的 用高效液相色譜法分析T2DM患者血漿遊離脂肪痠譜的變化.方法 收集武漢大學中南醫院體檢人群和住院患者,其中健康對照組94名、單純T2DM組101例、T2DM閤併高脂血癥組77例.取受試者空腹靜脈血,分離新鮮血漿,以正十七痠作為內標,以改良Doles法萃取遊離脂肪痠,溴苯乙酮作為柱前衍化劑,採用Dionex Summit高效液相色譜法分析繫統檢測血漿遊離脂肪痠譜,內標標準麯線法定量.結果 健康對照組、單純T2DM組與T2DM閤併高脂血癥組3組之間的總遊離脂肪痠濃度分彆為(355.63±100.35)、(421.21±200.83)、(473.04±213.40)μmol/L,3組之間差異有統計學意義(X2=13.08,P<0.01),不飽和遊離脂肪痠濃度依次為(206.29±61.94)、(218.11±110.28)、(240.94±116.79)μmol/L,3組之間差異無統計學意義(x2=2.17,P>0.05).單純T2DM組與健康對照組比較,總遊離脂肪痠升高[(421.21±200.83)μmol/L vs(355.63±100.35)μmol/L,x2=7.22,P<0.05],不飽和遊離脂肪痠濃度和健康對照組差異無統計學意義.T2DM閤併高脂血癥組與健康對照組比較,總遊離脂肪痠濃度升高[(473.04±213.40)μmol/L vs (355.63±100.35)μmol/L,x2=27.93,P<0.01].日間及日內RSD均<5%,最低檢測濃度為0.05～0.35 μmol/L,高、中、低濃度下的迴收率為96.4%～104.8%.結論 T2DM患者血漿總遊離脂肪痠濃度升高,以飽和遊離脂肪痠升高為主,不飽和遊離脂肪痠改變差異無統計學意義.可能與T2MD的髮生髮展有關,有望成為糖尿病脂代謝紊亂的臨床早期鑑測新指標.
목적 용고효액상색보법분석T2DM환자혈장유리지방산보적변화.방법 수집무한대학중남의원체검인군화주원환자,기중건강대조조94명、단순T2DM조101례、T2DM합병고지혈증조77례.취수시자공복정맥혈,분리신선혈장,이정십칠산작위내표,이개량Doles법췌취유리지방산,추분을동작위주전연화제,채용Dionex Summit고효액상색보법분석계통검측혈장유리지방산보,내표표준곡선법정량.결과 건강대조조、단순T2DM조여T2DM합병고지혈증조3조지간적총유리지방산농도분별위(355.63±100.35)、(421.21±200.83)、(473.04±213.40)μmol/L,3조지간차이유통계학의의(X2=13.08,P<0.01),불포화유리지방산농도의차위(206.29±61.94)、(218.11±110.28)、(240.94±116.79)μmol/L,3조지간차이무통계학의의(x2=2.17,P>0.05).단순T2DM조여건강대조조비교,총유리지방산승고[(421.21±200.83)μmol/L vs(355.63±100.35)μmol/L,x2=7.22,P<0.05],불포화유리지방산농도화건강대조조차이무통계학의의.T2DM합병고지혈증조여건강대조조비교,총유리지방산농도승고[(473.04±213.40)μmol/L vs (355.63±100.35)μmol/L,x2=27.93,P<0.01].일간급일내RSD균<5%,최저검측농도위0.05～0.35 μmol/L,고、중、저농도하적회수솔위96.4%～104.8%.결론 T2DM환자혈장총유리지방산농도승고,이포화유리지방산승고위주,불포화유리지방산개변차이무통계학의의.가능여T2MD적발생발전유관,유망성위당뇨병지대사문란적림상조기감측신지표.
Objective To analyze the plasma free fatty acid (FFA) composition in patients with T2DM. Methods All subjects were from Zhongnan hospital of Wuhan university, and they were divided into three groups: normal control ( n = 94 ), T2DM ( n = 101 ) and T2DM with hyperlipidemia ( n = 77 ). Fasting blood samples were taken from the participants, and plasma FFA were separated using a modified Doles method with the bromoacetophenone, pre-column-derivative. The quantitation of FFA was performed on were (355.63 ± 100. 35) μmol/L, (421.21 ± 200. 83 ) μ mol/L, ( 473.04 ± 213.40 ) μmol/L in healthy controls, T2DM group and T2DM with hyperlipidemia group, respectively. The significant differences were observed among the 3 groups(x2 = 13.08, P <0.01 ). However, there was no significant difference of UFA concentrations among the 3 groups [(206.29± 61.94) μ mol/L, (218.11 ± 110.28) μmol/L and ( 240.94 ± 116.79 ) μmol/L, x2 = 2.17, P > 0.05]. Compared to normal control [( 355.63 ± 100.35 )μmol/L], the FFA concentration[(421.21 ±200.83) μmol/L] in T2DM has significantly increased (x2 =FFA concentrations were higher in T2DM with hyperlipidemia [(473.04 ±213.40) μmol/L] (x2 =27.93,P <0.01 ). The RSD values for intra- and inter-day precision were less than 5%, and the minimal detection limits ranged from 0.05 μmol/L to 0.35 μmol/L The recoveries of high, intermediate and low-level materials were 96.4% -104.8%. Conclusions The total FFA concentration in T2DM has increased, most of which are saturated FFA. The unsaturated FFA has not significantly increased. They seem to be related to the development of T2DM, and might be a new biomarker for clinical monitoring of metabolic disorder of T2DM.
