CAJ | 학술논문

目的弥漫性轴索损伤( DAI)能导致伤后认知障碍.通过建立实验性 DAI动物模型,了解在 DAI伤后与认知功能关系密切海马生长抑素( Ss)样神经元的变化.方法采用 Marmarou打击装置建立 DAI动物模型,免疫组织化学染色以显示海马 Ss 样神经元.结果海马 Ss 样神经元在重伤组、轻伤组、对照组差异有非常显著性意义( F=21.789～ 125.205,P< 0.01);损伤后 2周组神经元减少与 1周组比较差异有显著性意义( t=1.833～ 6.038,P< 0.01, 0.05).结论 DAI后海马 Ss 样神经元的减少可能是伤后认知障碍,甚至是 DAI后植物生存的主要病理改变之一.伤后的迟发性细胞死亡在该种神经元的减少中起着重要作用.
목적미만성축색손상( DAI)능도치상후인지장애.통과건립실험성 DAI동물모형,료해재 DAI상후여인지공능관계밀절해마생장억소( Ss)양신경원적변화.방법채용 Marmarou타격장치건립 DAI동물모형,면역조직화학염색이현시해마 Ss 양신경원.결과해마 Ss 양신경원재중상조、경상조、대조조차이유비상현저성의의( F=21.789～ 125.205,P< 0.01);손상후 2주조신경원감소여 1주조비교차이유현저성의의( t=1.833～ 6.038,P< 0.01, 0.05).결론 DAI후해마 Ss 양신경원적감소가능시상후인지장애,심지시 DAI후식물생존적주요병리개변지일.상후적지발성세포사망재해충신경원적감소중기착중요작용.
Aim Numerous studies using experimental animals have also confirmed the important role of hippocampus somatostatinergic systems in cognitive processes.The purpose of this study was to see if a significant loss of hippcampus somatostatin - containing neurons of neurons after experiment diffuse axonal injury (DBI).Methods The present study adapted the model which Marmarou et al,developed a model of brain impact - acceleration injury were examined by adjusting the height of the falling weight to either 1 m(mild injury group) or 2 m(severe injury group).Morphological studies were performed in serial sections,using immunohistochemical SP staining method of somatostatin - containing neurons.Results The number of somatostatin - containing neurons in hippocamus were apparently decreased as compared with control group(P< 0.01).The number of neurons in severe injury group was aparently decreassed as compared with mild injury group(F=21.789-125.205,P< 0.01).The loss of neurons at two weeks was apparently different from those at one week after insult(t=1.833-6.038,P< 0.01 or P< 0.05).Conclusion A singnificant and apparently selective loss of hippocamus somatostatin - containing neurons following DAI is demonstrated in this study.This type of injury may be one of important pathological changes of cognitive dysfunction after diffuse axonal injury.Meanwhile,second injury plays a very important role in the loss of this type neurons.