CAJ | 학술논문

以天然纳米材料埃洛石为载体,通过物理吸附法对α-淀粉酶进行固定.利用红外光谱、扫描电镜、透射电镜等对埃洛石的结构和形貌特征进行测试与表征,同时对埃洛石纳米管固定化α-淀粉酶的条件及固定化酶的酶学性能进行了研究,并与游离酶进行了比较.结果表明:这种具有管状结构的埃洛石硅酸盐矿物是理想的酶载体,酶的固定化效率平均达到37.38%;所得的固定化α-淀粉酶4℃下保存15d后,酶活力仍保持90%以上;固定化α-淀粉酶的热稳定性也明显优于游离酶,连续7批次操作后仍保持56.2%的酶活力.
이천연납미재료애락석위재체,통과물리흡부법대α-정분매진행고정.이용홍외광보、소묘전경、투사전경등대애락석적결구화형모특정진행측시여표정,동시대애락석납미관고정화α-정분매적조건급고정화매적매학성능진행료연구,병여유리매진행료비교.결과표명:저충구유관상결구적애락석규산염광물시이상적매재체,매적고정화효솔평균체도37.38%;소득적고정화α-정분매4℃하보존15d후,매활력잉보지90%이상;고정화α-정분매적열은정성야명현우우유리매,련속7비차조작후잉보지56.2%적매활력.
Halloysite nanotubes, a new and economically viable material, were used to immobilize α-amylase by the physical adsorption method. The characteristics of the structure and morphology of the halloysite crystals were investigated by means of Fourier transform infrared spectroscopy, scanning electron microscope and transmission electron microscope. The properties of the immobilized enzyme were also investigated and compared with the free enzyme. It is demonstrated that the natural halloysite minerals are excellent supports for immobilizing enzymes. The immobilization efficiency of halloysite nanotubes is 37.38%, and higher than other supports under the same conditions. The thermal and storage stability of the immobilized α-amylase is much better than the free enzyme and the immobilized α-amylase could retain about 90% of the original activity for 15d at 4℃. After seven times repeated batch operation, 56.2% relative activity of the immobilized α-amylase was retained, which demonstrated high operational stability.