解放军医学杂志
解放軍醫學雜誌
해방군의학잡지
MEDICAL JOURNAL OF CHINESE PEOPLE'S LIBERATION ARMY
2008年
7期
841-843
,共3页
心肌缺血%再灌注损伤%一氧化氮%一氧化氮合酶%内皮缩血管肽%参附注射液
心肌缺血%再灌註損傷%一氧化氮%一氧化氮閤酶%內皮縮血管肽%參附註射液
심기결혈%재관주손상%일양화담%일양화담합매%내피축혈관태%삼부주사액
myocradial ischemia%reperfusion injury%nitric oxide%endothelial nitric oxide synthase%endothelin%shertFu injection
目的 观察兔心肌缺血再灌注(I/R)损伤中内皮功能的改变以及参附注射液(SFI)对其的影响和作用机制.方法 21只日本大耳白兔随机分为假手术对照组(A组)、心肌I/R模型组(B组)及心肌I/R+SFI治疗组(C组),每组7只.检测指标:①检测结扎前、缺血40min、再灌注40min三个时相点血清内皮型一氧化氮合酶(eNOS)、一氧化氮代谢产物(NOP)和血浆内皮素(ET)含量;②检测再灌注40min后心肌组织总超氧化物歧化酶(T-SOD)和丙二醛(MDA)含量;③电镜观察心肌超微结构.结果 ①B组与A组比较,缺血40min、再灌注40min血清eNOS、NOP明显降低,血浆ET显著增高,后两者呈显著负相关(均P<0.01);再灌注40min后心肌组织T-SOD明显降低,MDA明显增高(均P<0.01),NOP与T-SOD、ET与MDA均呈显著正相关(P<0.05),NOP与MDA、ET与T-SOD均呈显著负相关(P<0.05);心肌超微结构发生异常改变.②C组与B组比较,缺血40min、再灌注4min eNOS、NOP水平明显增高(均P<0.01);再灌注40min ET水平明显降低(P<0.01);再灌注40min后心肌组织T-SOD显著增高,MDA显著降低(均P<0.01);心肌超微结构的异常改变明显减轻.结论 SFI具有改善兔心肌I/R中自由基介导的内皮功能紊乱作用,并能减轻心肌I/R损伤.
目的 觀察兔心肌缺血再灌註(I/R)損傷中內皮功能的改變以及參附註射液(SFI)對其的影響和作用機製.方法 21隻日本大耳白兔隨機分為假手術對照組(A組)、心肌I/R模型組(B組)及心肌I/R+SFI治療組(C組),每組7隻.檢測指標:①檢測結扎前、缺血40min、再灌註40min三箇時相點血清內皮型一氧化氮閤酶(eNOS)、一氧化氮代謝產物(NOP)和血漿內皮素(ET)含量;②檢測再灌註40min後心肌組織總超氧化物歧化酶(T-SOD)和丙二醛(MDA)含量;③電鏡觀察心肌超微結構.結果 ①B組與A組比較,缺血40min、再灌註40min血清eNOS、NOP明顯降低,血漿ET顯著增高,後兩者呈顯著負相關(均P<0.01);再灌註40min後心肌組織T-SOD明顯降低,MDA明顯增高(均P<0.01),NOP與T-SOD、ET與MDA均呈顯著正相關(P<0.05),NOP與MDA、ET與T-SOD均呈顯著負相關(P<0.05);心肌超微結構髮生異常改變.②C組與B組比較,缺血40min、再灌註4min eNOS、NOP水平明顯增高(均P<0.01);再灌註40min ET水平明顯降低(P<0.01);再灌註40min後心肌組織T-SOD顯著增高,MDA顯著降低(均P<0.01);心肌超微結構的異常改變明顯減輕.結論 SFI具有改善兔心肌I/R中自由基介導的內皮功能紊亂作用,併能減輕心肌I/R損傷.
목적 관찰토심기결혈재관주(I/R)손상중내피공능적개변이급삼부주사액(SFI)대기적영향화작용궤제.방법 21지일본대이백토수궤분위가수술대조조(A조)、심기I/R모형조(B조)급심기I/R+SFI치료조(C조),매조7지.검측지표:①검측결찰전、결혈40min、재관주40min삼개시상점혈청내피형일양화담합매(eNOS)、일양화담대사산물(NOP)화혈장내피소(ET)함량;②검측재관주40min후심기조직총초양화물기화매(T-SOD)화병이철(MDA)함량;③전경관찰심기초미결구.결과 ①B조여A조비교,결혈40min、재관주40min혈청eNOS、NOP명현강저,혈장ET현저증고,후량자정현저부상관(균P<0.01);재관주40min후심기조직T-SOD명현강저,MDA명현증고(균P<0.01),NOP여T-SOD、ET여MDA균정현저정상관(P<0.05),NOP여MDA、ET여T-SOD균정현저부상관(P<0.05);심기초미결구발생이상개변.②C조여B조비교,결혈40min、재관주4min eNOS、NOP수평명현증고(균P<0.01);재관주40min ET수평명현강저(P<0.01);재관주40min후심기조직T-SOD현저증고,MDA현저강저(균P<0.01);심기초미결구적이상개변명현감경.결론 SFI구유개선토심기I/R중자유기개도적내피공능문란작용,병능감경심기I/R손상.
Objective To investigate the changes of endothelial function during myocardial ischemia and reperfusion (I/R) injury of rabbits, and the protection of ShenFu injection (SFI) for the injury, as well as the related mechanism. Methods Twenty one Japanese white rabbits were randomly divided into 3 groups (7 each): sham operated group (A), I/R group (B) and SFI-treated I/R group (C). The activity of serum endothelial nitric oxide synthase(eNOS), the concentrations of serum nitric oxide products (NOP) and plasma endothelin (ET) before ligation of coronary artery, 40min after ischemia and 40min after reperfusion were determined. The concentration of total superoxide dismutase (T-SOD) and malonyl-dialdehyde (MDA) in myocardial tissue were determined. Ultrastructure of myocardial cell was observed by electro microscope. Results 1) Serum eNOS and NOP decreased and plasma ET increased ingroup B than that in group A after 40min of ischemia (P<0.01), even more significant after 40min of reperfusion. NOP was correlated positively with ETafter 40min of ischemia (P<0. 05) and after 40min of reperfusion ( P<0. 01). In 40min after reperfusion, T-SOD was decreased and MDA was increased ingroup B than that in group A, significantly (P<0. 01). NOP were correlated positively with T-SOD, while were correlated negatively with MDA (P<0.05). ET was correlated positively with MDA, while was correlated negatively with T-SOD (P<0.05). Ultrastructure of myocardial cell changed abnormally. 2) SFI can increase eNOS and NOP after 40min ischemia and 40min reperfusion, and decrease ET after 40min reperfusion, significantly (P<0. 01). SFI can increase T-SOD and decrease MDA significantly (P<0. 01), the abnormality of myocardial cells can be relieved obviously. Conclusion SFI can improve the endothelial function in which free radicals are involved, and prevent myocardial tissue from I/R injury.
