CAJ | 학술논문

[目的] 采用反向斑点杂交技术对拉米夫定治疗后的慢性乙型肝炎患者血清中HBV YMDD基序变异进行检测,并对其临床应用进行方法学评价.[方法] 提取242例慢性乙型肝炎患者血清中HBV DNA,经聚合酶链反应后进行膜条杂交并同测序比较分析检测结果的一致性,随后对该方法进行灵敏度和混合感染检测能力的评价.[结果] 242例样本检测结果有236例与测序结果相符,反向斑点杂交检测结果同测序结果的符合率达97.5%;混合型感染58例,占样品总数的24%.反向斑点杂交技术检测HBV YMDD基序变异的灵敏度达103 IU/mL,在病毒混合感染群体中能检测出约占10%的突变型病毒株.[结论] 反向斑点杂交技术检测HBV YMDD基序变异具有简单、准确、经济实用的特点,具有很好的临床应用前景.
[목적] 채용반향반점잡교기술대랍미부정치료후적만성을형간염환자혈청중HBV YMDD기서변이진행검측,병대기림상응용진행방법학평개.[방법] 제취242례만성을형간염환자혈청중HBV DNA,경취합매련반응후진행막조잡교병동측서비교분석검측결과적일치성,수후대해방법진행령민도화혼합감염검측능력적평개.[결과] 242례양본검측결과유236례여측서결과상부,반향반점잡교검측결과동측서결과적부합솔체97.5%;혼합형감염58례,점양품총수적24%.반향반점잡교기술검측HBV YMDD기서변이적령민도체103 IU/mL,재병독혼합감염군체중능검측출약점10%적돌변형병독주.[결론] 반향반점잡교기술검측HBV YMDD기서변이구유간단、준학、경제실용적특점,구유흔호적림상응용전경.
[Objective] To detect HBV YMDD motif mutants using RDB hybridization assay in lamivudine treated patients with chronic hepatitis B virus infection,as well as to evaluate the detection capability for clinical application.[Method] HBV DNA was extracted from serum for a total of 242 cases,after the PCR amplification,the hybridization was performed.By comparing the RDB assay results to sequence analysis,the concordant results were analyzed.The sensitivity and detection capability for mixed infection samples are also evaluated.[Results] There are 236 of concordant results for RDB assay and sequencing were obtained in a total of 242 cases,accounting for 97.5%.For all of the cases,there are 58 cases with coexisting mutant viruses in wild type viruses,accounting for 24%.The sensitivity of RDB hybridization assay for HBV YMDD motif mutants was 103 IU/mL,and approximately 10% mutant type strains can be detected from a mixed infection sample.[Conclusion] The RDB hybridization assay for HBV YMDD motif mutants is a simple,accurate,and economic method and it may be a promising tool for clinical application.