生物技术通报
生物技術通報
생물기술통보
BIOTECHNOLOGY BULLETIN
2009年
7期
166-170
,共5页
β-甘露聚糖酶%假单孢菌%分离%鉴定%酶活性
β-甘露聚糖酶%假單孢菌%分離%鑒定%酶活性
β-감로취당매%가단포균%분리%감정%매활성
β-mannanase Pseudomonas sp. Isolation Identification Activity
以采集的土壤样品为试验材料,经过富集培养及分离纯化,筛选出2株产β-甘露聚糖酶的菌株.采用摇瓶培养分别测定其酶活力,其中1株菌株酶活力较高,酶活力达50.36 U/ml.生理生化性质鉴定及16S rDNA序列相似性结果表明该菌为假单孢菌(Pseudomonas sp.).产酶的最适条件研究发现,1 g/100 ml玉米粉,2 g/100 ml魔芋粉,pH6.0,32℃为最优产酶条件,酶活力达185.37 U/ml,是优化前的3.68倍.该菌产酶迅速,培养12 h后已达产酶高峰.粗酶的性质研究发现,该酶是一种酸性的甘露聚糖酶,作用的最适pH为4.0,最适反应温度为55℃;该酶的稳定性较好,在pH4.0～6.0、温度为40～55℃保持较好的稳定性.
以採集的土壤樣品為試驗材料,經過富集培養及分離純化,篩選齣2株產β-甘露聚糖酶的菌株.採用搖瓶培養分彆測定其酶活力,其中1株菌株酶活力較高,酶活力達50.36 U/ml.生理生化性質鑒定及16S rDNA序列相似性結果錶明該菌為假單孢菌(Pseudomonas sp.).產酶的最適條件研究髮現,1 g/100 ml玉米粉,2 g/100 ml魔芋粉,pH6.0,32℃為最優產酶條件,酶活力達185.37 U/ml,是優化前的3.68倍.該菌產酶迅速,培養12 h後已達產酶高峰.粗酶的性質研究髮現,該酶是一種痠性的甘露聚糖酶,作用的最適pH為4.0,最適反應溫度為55℃;該酶的穩定性較好,在pH4.0～6.0、溫度為40～55℃保持較好的穩定性.
이채집적토양양품위시험재료,경과부집배양급분리순화,사선출2주산β-감로취당매적균주.채용요병배양분별측정기매활력,기중1주균주매활력교고,매활력체50.36 U/ml.생리생화성질감정급16S rDNA서렬상사성결과표명해균위가단포균(Pseudomonas sp.).산매적최괄조건연구발현,1 g/100 ml옥미분,2 g/100 ml마우분,pH6.0,32℃위최우산매조건,매활력체185.37 U/ml,시우화전적3.68배.해균산매신속,배양12 h후이체산매고봉.조매적성질연구발현,해매시일충산성적감로취당매,작용적최괄pH위4.0,최괄반응온도위55℃;해매적은정성교호,재pH4.0～6.0、온도위40～55℃보지교호적은정성.
Two strains which produced β-mannanase were isolated from soil by enriching and isolating. A strain with the higher enzyme activity of 50.37 U/ml was obtained after the enzyme activity assay. The strain was identified as Pseudomonas sp. by morphological and physiological characteristics analysis and 16S rDNA BLAST. The optimal conditions for producing β-mannanase by the strain were determined 1 g/l00 ml maizena, 2 g/100 ml konjak powder, pH6.0 and the optimal temperature was 32 ℃, under which the activity of β-mannanase was up to 185.37 U/ml,3.68 times as before. The strain which rapidly produced β-mannanase showed the highest β-mannanase activity after culturing for 12 h. The study of the enzymatic character of the β-mannanase revealed that it was the acidic β-mannanase, and the optimal condition of the enzyme was pH4.0 and 60℃, respectively. The activity of β-mannanase was stable at pH4.0～6.0 and 40～55℃.
