CAJ | 학술논문

我们已从文蛤肉中提取得到1种具有抗癌活性的多肽,命名为Mer2.本文报道Mer2对体外培养的肺癌A549细胞的生物学效应.采用溴化二苯偶氮盐(MTT)法检测Mer2对肺癌A549细胞的抑制效果,并应用细胞形态观察法、Hoechst荧光染色和流式细胞术等方法,探讨了Mer2对细胞形态、细胞周期的影响.结果表明,Mer2对体外培养的肺癌A549细胞的生长有较强的抑制作用,对作用含量和时间均具有一定的依赖性.Mer2使细胞形态发生明显变化,明显地出现凋亡峰,但并没有出现明显细胞周期阻滞现象.这表明文蛤多肽可能通过诱导细胞凋亡从而抑制癌细胞的生长.
아문이종문합육중제취득도1충구유항암활성적다태,명명위Mer2.본문보도Mer2대체외배양적폐암A549세포적생물학효응.채용추화이분우담염(MTT)법검측Mer2대폐암A549세포적억제효과,병응용세포형태관찰법、Hoechst형광염색화류식세포술등방법,탐토료Mer2대세포형태、세포주기적영향.결과표명,Mer2대체외배양적폐암A549세포적생장유교강적억제작용,대작용함량화시간균구유일정적의뢰성.Mer2사세포형태발생명현변화,명현지출현조망봉,단병몰유출현명현세포주기조체현상.저표명문합다태가능통과유도세포조망종이억제암세포적생장.
An anticancer peptide named as mer2 was purified from Meretrix meretrix.In this paper,we investigated its biological effect on the lung adenocarcinoma A549 cells.The effect of Mer2 on the growth of A549 cells was detected by MTT assay,and the morphology and the cell-cycle of cells were detected by optical microscope,Hoechst staining and flow cytometry.The results showed that Mer2 inhibited the growth of A549 cells in a dose-and time-dependent manner and cell morphology changed distinctly,showing an obvious apoptotsis properties.It indicated that the inhibitory effect of the peptide on cells had a relationship with the cell apoptotsis.