CAJ | 학술논문

目的探讨低温氧合血+Celsior液微流量持续灌注对大鼠右心的保护效果.方法 Wistar大鼠切取全心,随机分为3组,实验组的心脏以4℃氧合血+Celsior液微流量(1 mL/h)持续灌注低温保存4 h;对照组以4℃Celsior液微流量持续灌注低温保存4 h;单纯冷保存组以4℃Celsior液单纯浸泡保存4 h.保存后的鼠心用Langen-dorff装置灌注,切除右心耳,将一球囊放入右心室.测定右心室血流动力学指标;高效液相色谱仪测定ATP含量;光镜和电镜观察心肌组织的形态学改变.结果灌注30 min时,实验组的右心室收缩压(RVSP)为(26.64±3.3)mmHg,右室舒张压(RVDP)为(19.07±3.23)mmHg,右心室压力微分(±dp/dt)为(752±77)mmH/s;对照组的RVSP为(23.98±4.02)mmHg,RVDP为(11.31±2.46)mmHg,±dp/dt为(548±103)mmHg/s;单纯冷保存组的RVSP为(22.38±3.65)mmHg,RVDP为(9.43±2.44)mmHg,±dp/dt为(457±151)mmHg/s,实验组的血流动力学指标优于对照组和单纯冷保存组(P<0.05).实验组心肌细胞的ATP含量为(1.225±0.316)μmol/L,对照组为(0.852±0.303)μmol/L,单纯冷冻保存组为(0.587±0.232)μmol/L,实验组ATP含量明显高于对照组和单纯冷保存组(P<0.05).实验组的组织学损害轻于对照组和单纯冷保存组.结论低温氧合血+Celsior液微流量持续灌注对大鼠右心有保护效果.
목적 탐토저온양합혈+Celsior액미류량지속관주대대서우심적보호효과.방법 Wistar대서절취전심,수궤분위3조,실험조적심장이4℃양합혈+Celsior액미류량(1 mL/h)지속관주저온보존4 h;대조조이4℃Celsior액미류량지속관주저온보존4 h;단순랭보존조이4℃Celsior액단순침포보존4 h.보존후적서심용Langen-dorff장치관주,절제우심이,장일구낭방입우심실.측정우심실혈류동역학지표;고효액상색보의측정ATP함량;광경화전경관찰심기조직적형태학개변.결과 관주30 min시,실험조적우심실수축압(RVSP)위(26.64±3.3)mmHg,우실서장압(RVDP)위(19.07±3.23)mmHg,우심실압력미분(±dp/dt)위(752±77)mmH/s;대조조적RVSP위(23.98±4.02)mmHg,RVDP위(11.31±2.46)mmHg,±dp/dt위(548±103)mmHg/s;단순랭보존조적RVSP위(22.38±3.65)mmHg,RVDP위(9.43±2.44)mmHg,±dp/dt위(457±151)mmHg/s,실험조적혈류동역학지표우우대조조화단순랭보존조(P<0.05).실험조심기세포적ATP함량위(1.225±0.316)μmol/L,대조조위(0.852±0.303)μmol/L,단순냉동보존조위(0.587±0.232)μmol/L,실험조ATP함량명현고우대조조화단순랭보존조(P<0.05).실험조적조직학손해경우대조조화단순랭보존조.결론 저온양합혈+Celsior액미류량지속관주대대서우심유보호효과.
Objective To study the effects of continuous hypothermal oxygenated blood with Celsior cardiac preservation solution(CPS)microperfusion on rat right heart preservation.Methods Wistar rats were randomly divided into 3 groups and the whole heart was obtained.The isolated rat hearts were continuously microperfused by oxygenated blood with Celsior at 4℃ in experimental group,those in control group were mieroperfused with Celsior solution and those in solution group were preserved with Celsior solution for 4 hours respectively.Right atrial resection was performed before insertion of a latex balloon througb the atrium into the ventricle.After preservation,the hemodynamic indexes of rat heart were measured using Langendorff system,including right ventricular systolic pressure(RVSP),right ventricular diastolic pressure(RVDP)and the rate of the right ventricular pressure change(±dp/dt);then the contents of adenosine triphosphate(ATP)were determined by high-performance liquid chromatography and myocardium structures were observed under an electric microscope or by H-E.Results After 30 min perfusion of Langendorff system,the RVSP,RVDP and±dp/dt were(26.64±3.32)mmHg,(29.07±3.23)mmHg and(752±77)mmHg/s respectively in experimental group;(23.98±4.02)mmHg,(11.31±2.46)mmHg and(548±103)mmHg/s respectively in control group;(22.38±3.65)mmHg,(9.43±2.44)mmHg and(457±151)mmHg/s respectively in solution group.The hemodvnamic indexes in experimental group were superior to those in control and solution group(P<0.05).The contents of ATP in experimental,control and solution group were(1.225±0.316),(0.852±0.303)and(0.587±0.232)μmol/L respectively.The contents of ATP in experimental grouD were significantly than those in control and solution group(P<0.05).The changes of myocardium uhrastruc ture in experimental group were milder than those in control and solution group.ConclusionContinuous oxygenated blood with Celsior microperfusion can improve the hypothermic preservation effects on rat right heart.