CAJ | 학술논문

以蚕豆(Vicia faba)为材料,利用激光共聚焦显微技术和分光光度技术,结合药理学实验,探讨硫化氢(hydrogen sulphide,H2S)和过氧化氢(hydrogen peroxide,H2O2)在茉莉酸(jasmonic acid,JA)调控气孔运动信号转导中的作用.结果表明,H2S合成抑制剂氨氧基乙酸(aminooxyaceticacid,AOA)、羟胺(hydroxylamine,NH2OH)、丙酮酸钾(potasium pyruvate,C3H3KO3)和氨水(ammonia,NH3),H2O2清除剂抗坏血酸(ascorbic acid,AsA),合成抑制剂水杨羟肟酸(salicylhydroxamic acid,SHAM)、二苯基碘(diphenylene iodonium,DPI)均可逆转JA诱导的气孔关闭效应.JA能够明显提高蚕豆叶片及保卫细胞中的H2O2水平、H2S含量和L-/D-半胱氨酸脱巯基酶活性;H2S合成抑制剂可抑制JA引起的叶片H2S含量的增加;而H2O2清除剂则可减弱JA对H2S含量变化和L-/D-半胱氨酸脱巯基酶活性的诱导效应.以上结果表明H2S和H2O2均参与了JA诱导的蚕豆气孔关闭,且H2S(主要由L-/D-半胱氨酸脱巯基酶合成)可能作为H2O2的下游组分参与调控这一信号转导过程.
이잠두(Vicia faba)위재료,이용격광공취초현미기술화분광광도기술,결합약이학실험,탐토류화경(hydrogen sulphide,H2S)화과양화경(hydrogen peroxide,H2O2)재말리산(jasmonic acid,JA)조공기공운동신호전도중적작용.결과표명,H2S합성억제제안양기을산(aminooxyaceticacid,AOA)、간알(hydroxylamine,NH2OH)、병동산갑(potasium pyruvate,C3H3KO3)화안수(ammonia,NH3),H2O2청제제항배혈산(ascorbic acid,AsA),합성억제제수양간우산(salicylhydroxamic acid,SHAM)、이분기전(diphenylene iodonium,DPI)균가역전JA유도적기공관폐효응.JA능구명현제고잠두협편급보위세포중적H2O2수평、H2S함량화L-/D-반광안산탈구기매활성;H2S합성억제제가억제JA인기적협편H2S함량적증가;이H2O2청제제칙가감약JA대H2S함량변화화L-/D-반광안산탈구기매활성적유도효응.이상결과표명H2S화H2O2균삼여료JA유도적잠두기공관폐,차H2S(주요유L-/D-반광안산탈구기매합성)가능작위H2O2적하유조분삼여조공저일신호전도과정.