白血病·淋巴瘤
白血病·淋巴瘤
백혈병·림파류
JOURNAL OF LEUKEMIA & LYMPHOMA
2009年
8期
452-454
,共3页
白血病%树突细胞%T淋巴细胞%细胞毒性%钙离子载体
白血病%樹突細胞%T淋巴細胞%細胞毒性%鈣離子載體
백혈병%수돌세포%T림파세포%세포독성%개리자재체
Leukemia%Dendritic cells%T-lymphocyte%cytotoxic%Calcium ionophore
目的 研究白血病来源树突状细胞体外诱导的有效方法;观察不同抗原诱导的特异性细胞毒性T淋巴细胞(CTL)的抗白血病效应.方法 分离白血病患者骨髓单个核细胞,经钙离子载体A23187诱导分化.将弱酸洗脱抗原、低渗抗原分别冲击树突状细胞,96 h后树突状细胞与T细胞共同培养,采用MTT法比较CTL细胞对白血病细胞的杀伤活性.结果 骨髓来源的单个核细胞经过100ng/ml的GM-CSF、500 ng/ml钙离子载体A23187成功诱导为树突状细胞,倒置显微镜下具有树突状细胞的典型形态,流式细胞术测定其CDla、CD83,表达较诱导前明显升高,差异有统计学意义(P<0.01).弱酸洗脱法获得抗原冲击树突状细胞致敏T细胞后杀伤白血病的能力最高,未负载抗原的树突状细胞致敏T细胞杀伤白血病细胞的能力最低,两者差异具有统计学意义(P<0.01).结论 白血病来源的骨髓单个核细胞经GM-CSF、钙离子载体A23187能够成功诱导为树突状细胞;弱酸洗脱后获得的抗原冲击树突状细胞致敏T细胞能够获得更强的杀伤白血病细胞的能力.
目的 研究白血病來源樹突狀細胞體外誘導的有效方法;觀察不同抗原誘導的特異性細胞毒性T淋巴細胞(CTL)的抗白血病效應.方法 分離白血病患者骨髓單箇覈細胞,經鈣離子載體A23187誘導分化.將弱痠洗脫抗原、低滲抗原分彆遲擊樹突狀細胞,96 h後樹突狀細胞與T細胞共同培養,採用MTT法比較CTL細胞對白血病細胞的殺傷活性.結果 骨髓來源的單箇覈細胞經過100ng/ml的GM-CSF、500 ng/ml鈣離子載體A23187成功誘導為樹突狀細胞,倒置顯微鏡下具有樹突狀細胞的典型形態,流式細胞術測定其CDla、CD83,錶達較誘導前明顯升高,差異有統計學意義(P<0.01).弱痠洗脫法穫得抗原遲擊樹突狀細胞緻敏T細胞後殺傷白血病的能力最高,未負載抗原的樹突狀細胞緻敏T細胞殺傷白血病細胞的能力最低,兩者差異具有統計學意義(P<0.01).結論 白血病來源的骨髓單箇覈細胞經GM-CSF、鈣離子載體A23187能夠成功誘導為樹突狀細胞;弱痠洗脫後穫得的抗原遲擊樹突狀細胞緻敏T細胞能夠穫得更彊的殺傷白血病細胞的能力.
목적 연구백혈병래원수돌상세포체외유도적유효방법;관찰불동항원유도적특이성세포독성T림파세포(CTL)적항백혈병효응.방법 분리백혈병환자골수단개핵세포,경개리자재체A23187유도분화.장약산세탈항원、저삼항원분별충격수돌상세포,96 h후수돌상세포여T세포공동배양,채용MTT법비교CTL세포대백혈병세포적살상활성.결과 골수래원적단개핵세포경과100ng/ml적GM-CSF、500 ng/ml개리자재체A23187성공유도위수돌상세포,도치현미경하구유수돌상세포적전형형태,류식세포술측정기CDla、CD83,표체교유도전명현승고,차이유통계학의의(P<0.01).약산세탈법획득항원충격수돌상세포치민T세포후살상백혈병적능력최고,미부재항원적수돌상세포치민T세포살상백혈병세포적능력최저,량자차이구유통계학의의(P<0.01).결론 백혈병래원적골수단개핵세포경GM-CSF、개리자재체A23187능구성공유도위수돌상세포;약산세탈후획득적항원충격수돌상세포치민T세포능구획득경강적살상백혈병세포적능력.
Objective To explore the effective method for in vitro culture of the dendritic cells(DCs) and the specific anti-leukemic cell effect mediated by dendritic cells pulsed with acute myelogenous leukemia antigen. Methods Bone marrow mononucleas cells (BMMNCs) isolated from AML patients were induced to undergo differentiation with 500 ng/ml A23187 and pulsed with AML antigen. After 96 h, DCs and T cells were co-cultured for 5 to 7 days. The cytotoxic activity of cytotoxic T lymphocyte(CTL) to AML were detected with MTT colorimetry. Results BMMNCs isolated from AML patients treated with 100 ng/ml rhGM-CSF in combination with 500 ng/ml A23187 for 96 h exhibited typical morphology of DCs with rapidly increased expression of CD1a and CD83 (P<0.01). Dendritic cells pulsed with acid eluted tumor antigen peptides group displayed the strongest cytotoxic activity of CTL to AML. There was a significant difference between two groups(P<0.01). Conclusion BMMNCs isolated from AML patients can be successfully induced to DCs with rhGM-CSF and A23187 and dendritic cells pulsed with acid eluted tumor antigen peptides group had the strongest cytotoxic activity of CTL to AML.
