CAJ | 학술논문

目的研究呼出气冷凝液(EBC)技术用于呼吸道表面液体层采样以检测抗菌药物在其中分布的可行性.方法 6名健康受试者单剂空腹口服左氧氟沙星片剂500 mg后,分别于给药前和给药后1、2、4、8、12、24 h收集EBC,以高效液相-荧光检测法测定其中的药物浓度.结果 6名受试者单剂口服左氧氟沙星后在各时间点的中位浓度(最大值,最小值)依次为0.000(0.000,0.000)、0.000(0.000,0.000)、0.000(0.000,0.230)、0.000(0.000,0.478)、0.000(0.000,0.173)、0.015(0.000,0.095)mg/L,其中3名受试者在服药后24 h内始终未测得药物浓度,其他3名受试者在服药后4 h及以后才测得药物浓度,且并未呈现其药物浓度随时间动态趋势.受试者试验期间无不良事件发生.结论虽然EBC技术用于呼吸道表面液体层采样简便、安全,但是现有收集方法稀释度高,使其在测定呼吸道表面液体层中抗菌药物分布方面缺乏可行性.
목적 연구호출기냉응액(EBC)기술용우호흡도표면액체층채양이검측항균약물재기중분포적가행성.방법 6명건강수시자단제공복구복좌양불사성편제500 mg후,분별우급약전화급약후1、2、4、8、12、24 h수집EBC,이고효액상-형광검측법측정기중적약물농도.결과 6명수시자단제구복좌양불사성후재각시간점적중위농도(최대치,최소치)의차위0.000(0.000,0.000)、0.000(0.000,0.000)、0.000(0.000,0.230)、0.000(0.000,0.478)、0.000(0.000,0.173)、0.015(0.000,0.095)mg/L,기중3명수시자재복약후24 h내시종미측득약물농도,기타3명수시자재복약후4 h급이후재측득약물농도,차병미정현기약물농도수시간동태추세.수시자시험기간무불량사건발생.결론 수연EBC기술용우호흡도표면액체층채양간편、안전,단시현유수집방법희석도고,사기재측정호흡도표면액체층중항균약물분포방면결핍가행성.
Objective To investigate the feasibility of exhaled breath condensate (EBC) as a sampling technique to evaluate the antibiotic distribution in the airway surface liquid. Methods Six healthy volunteers were enrolled. Before and after 1,2,4,8, 12, and 24 hours of orally taking 500 mg levofloxacin, EBC was collected and the level of levofloxacin was measured by high performance liquid chromatography-fluorescence detection. Results The drug concentration in EBC at each time point after oral administration was 0. 000 (0.000, 0.000), 0.000 (0.000, 0.000), 0.000 (0.000,0.230), 0.000 (0. 000,0. 478), 0. 000 (0. 000,0.173), and 0. 015 (0. 000,0. 095) mg/L. Levofloxacin was undetectable in three of all the six subjects, and that was detectable in the other three subjects after four hours of taking levofloxacin,but there was no dynamic trend of drug concentration with time. No adverse effect was observed during the experiment. Conclusions EBC technology is simple and safe for sampling in airway surface liquid, but the consideration of dilution and low levels of drug concentration preclude EBC to become a promising sampling technique on studying the antibiotic distribution in airway surface liquid.