中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2008年
12期
725-728
,共4页
龚邦东%裴彬%杜德林%李仲平%李宇舟
龔邦東%裴彬%杜德林%李仲平%李宇舟
공방동%배빈%두덕림%리중평%리우주
转化生长因子β%多态性,单核苷酸%肝炎,乙型,慢性%基因型%聚合酶链反应
轉化生長因子β%多態性,單覈苷痠%肝炎,乙型,慢性%基因型%聚閤酶鏈反應
전화생장인자β%다태성,단핵감산%간염,을형,만성%기인형%취합매련반응
Transforming growth factor beta%Polymorphism,single nucleotide%Hepatitis B,chronic%Genotypes%Polymerase chain reaction
目的 探讨转化生长因子(TGF)-β1单核苷酸多态性(SNP)与慢性HBV感染的关系.方法 采用PCR方法,对74例慢性乙型肝炎患者、41例乙型肝炎肝硬化患者和41例健康人外周血细胞基因组DNA的TGF-β1基因中三个SNP位点(rs2241715、rs2241716、rs4803455)进行基因分型.数据分析采用χ2或Fisher精确概率法检验.结果 健康人的 rs2241715基因型及等位基因频率与慢性乙型肝炎和肝硬化患者相比,差异有统计学意义(χ2=11.419,P＜0.01或χ2=6.218χ2=5.961;P＜0.05),rs2241715 T/T基因型的个体患慢性乙型肝炎和乙型肝炎肝硬化的相对危险度分别为其他型的2.974倍(95%CI=1.209～7.314,P=0.018)和3.228倍(95%CI=1.201～8.675,P=0.020),rs2241716和rs4803455位点则无上述相关性.结论 TGF-131基因的rs2241715 SNP位点T/T基因型可能与慢性HBV感染密切相关.
目的 探討轉化生長因子(TGF)-β1單覈苷痠多態性(SNP)與慢性HBV感染的關繫.方法 採用PCR方法,對74例慢性乙型肝炎患者、41例乙型肝炎肝硬化患者和41例健康人外週血細胞基因組DNA的TGF-β1基因中三箇SNP位點(rs2241715、rs2241716、rs4803455)進行基因分型.數據分析採用χ2或Fisher精確概率法檢驗.結果 健康人的 rs2241715基因型及等位基因頻率與慢性乙型肝炎和肝硬化患者相比,差異有統計學意義(χ2=11.419,P＜0.01或χ2=6.218χ2=5.961;P＜0.05),rs2241715 T/T基因型的箇體患慢性乙型肝炎和乙型肝炎肝硬化的相對危險度分彆為其他型的2.974倍(95%CI=1.209～7.314,P=0.018)和3.228倍(95%CI=1.201～8.675,P=0.020),rs2241716和rs4803455位點則無上述相關性.結論 TGF-131基因的rs2241715 SNP位點T/T基因型可能與慢性HBV感染密切相關.
목적 탐토전화생장인자(TGF)-β1단핵감산다태성(SNP)여만성HBV감염적관계.방법 채용PCR방법,대74례만성을형간염환자、41례을형간염간경화환자화41례건강인외주혈세포기인조DNA적TGF-β1기인중삼개SNP위점(rs2241715、rs2241716、rs4803455)진행기인분형.수거분석채용χ2혹Fisher정학개솔법검험.결과 건강인적 rs2241715기인형급등위기인빈솔여만성을형간염화간경화환자상비,차이유통계학의의(χ2=11.419,P＜0.01혹χ2=6.218χ2=5.961;P＜0.05),rs2241715 T/T기인형적개체환만성을형간염화을형간염간경화적상대위험도분별위기타형적2.974배(95%CI=1.209～7.314,P=0.018)화3.228배(95%CI=1.201～8.675,P=0.020),rs2241716화rs4803455위점칙무상술상관성.결론 TGF-131기인적rs2241715 SNP위점T/T기인형가능여만성HBV감염밀절상관.
Objective To explore the genetic impact of three newly discovered single nueleotide polymorphism (SNP) sites of the transforming growth factor (TGF)-β1 gene on the susceptibility of the chronic hepatitis B virus (HBV) infection. Methods Genome DNA was extracted from the peripheral blood samples of 115 cases suffering from the chronic HBV infection (74 chronic hepatitis B, 41 cirrhosis) as well as 41 healthy volunteers. Thereafter, genotyping of rs2241715, rs2241716 and rs4803455 sites of the TGF-β1 gene was performed by genotype-specific polymerase chain reaction (PCR) analysis. The data were analyzed by the ehi square test and Fisher exact test. Results There was a significant difference of rs2241715 genotypes and allele frequencies between healthy volunteers and patients with chronic hepatitis B and cirrhosis (χ2 = 11.419, P＜0.01 and χ2 = 6.218, χ2 = 5.961,P＜0.05,respectively). Interestingly, the risk relative of subjects with T/T genotype suffered from chronic hepatitis B (OR = 2. 974, 95% CI = 1.209 - 7. 314, P = 0.018) and cirrhosis (OR = 3.228, 95%CI=1.201-8.675, P=0.020) was dramatically higher than that in patients with T/G or G/G genotypes. Conclusion The TGF-β1 rs2241715 T/T genotype appears to be associated with the chronic HBV infection.
