CAJ | 학술논문

目的观察不同部位伤害性刺激下大鼠脑功能性磁共振成像的变化.方法健康清洁级SD大鼠,体重300～450 g,雌雄不拘,采用Y型迷宫筛选出对电刺激反应较敏感的48只大鼠,采用随机数定表法,将其随机分为2组(n=24):刺激鼠尾组(T组)和刺激左前爪组(LF组).T组于鼠尾给予伤害性电刺激,LF组于左前爪给予伤害性电刺激,刺激期间行脑功能磁共振成像扫描,采用统计参数图2软件行图像分析.结果 T组中枢主要的激活脑区包括:初级感觉皮层、次级感觉皮质、后扣带回皮质、杏仁核和腹后外侧丘脑核;LF组中枢主要的激活脑区包括:右侧伏膈核、右侧初级感觉皮层、右侧腹后外侧丘脑核和右侧后扣带回皮质.结论不同部位伤害性刺激下大鼠脑功能性磁共振成像在中枢激活的脑区不同.
목적 관찰불동부위상해성자격하대서뇌공능성자공진성상적변화.방법 건강청길급SD대서,체중300～450 g,자웅불구,채용Y형미궁사선출대전자격반응교민감적48지대서,채용수궤수정표법,장기수궤분위2조(n=24):자격서미조(T조)화자격좌전조조(LF조).T조우서미급여상해성전자격,LF조우좌전조급여상해성전자격,자격기간행뇌공능자공진성상소묘,채용통계삼수도2연건행도상분석.결과 T조중추주요적격활뇌구포괄:초급감각피층、차급감각피질、후구대회피질、행인핵화복후외측구뇌핵;LF조중추주요적격활뇌구포괄:우측복격핵、우측초급감각피층、우측복후외측구뇌핵화우측후구대회피질.결론 불동부위상해성자격하대서뇌공능성자공진성상재중추격활적뇌구불동.
Objective To investigate the change in brain functional magnetic resonance imaging (fMRI)during noxious electric stimu lation of forepaw and tail in rats.Methods Forty-eight healthy SD rats of both sexes weighing 300-450 g which responded sensitively to electrical stimulation as shown by Y-maze were randomly divided into 2 groups ( n =24 each) receiving nociceptive electric stimulation of left forepaw (group LF) and tail (group T) respectively.The animals were anesthetized with intraperitoneal 10％ chloral hydrate 250-300 mg/kg.Brain fMRI was performed at 30 min after disappearance of righting reflex in both groups when electric stimulations were delivered intermittently to forepaw or tail.The images were analyzed with SPM2 software.Results Primary and secondary somatosensory cortex,posterior cingutate cortex,amygdaloid nucleus and ventral posterolateral thalamic nucleus were activated in group T.Right accumbens nucleus,right primary somatosensory cortex,right ventral posterolateral thalamic nucleus and right posterior cingutate cortex were activated in group LF.Conclusion Different brain areas are activated by noxious electric stimulation of different parts of body as shown by brain fMRI.