CAJ | 학술논문

目的筛查与分析急性白血病特异性的磷酸化蛋白质或多肽.方法对16例初治急性淋巴细胞白血病(ALL)和20例急性髓系白血病(AML)患者骨髓单个核细胞行蛋白多肽抽提及纯化,采用免疫沉淀及结合高效液相质谱-串联质谱分析,筛查特异性分子标志物.结果 ①非受体型酪氨酸蛋白激酶家族的Fyn、Yes和Src等在急性白血病中广泛表达；②非受体型酪氨酸蛋白激酶家族的Abl/iso1、Abl,非受体型丝氨酸/苏氨酸蛋白激酶家族的Bcr、JNK2、JNK2 iso2,结合器/支架类的Cas-L、Cbl、CrkL CENTD1( Centaurin deha1) ZO2,转录调节因子GFR-1及磷酸酯酶SHIP-2,这些磷酸化的蛋白见于Ph染色体阳性ALL,基本不表达于其他ALL;③Hck、Lyn和Fgr选择性地与AML相关(M3除外).结论 ALL和AML存在相对特异的磷酸化多肽,为急性白血病的诊断及治疗提供了潜在的分子靶位.
목적 사사여분석급성백혈병특이성적린산화단백질혹다태.방법 대16례초치급성림파세포백혈병(ALL)화20례급성수계백혈병(AML)환자골수단개핵세포행단백다태추제급순화,채용면역침정급결합고효액상질보-천련질보분석,사사특이성분자표지물.결과 ①비수체형락안산단백격매가족적Fyn、Yes화Src등재급성백혈병중엄범표체；②비수체형락안산단백격매가족적Abl/iso1、Abl,비수체형사안산/소안산단백격매가족적Bcr、JNK2、JNK2 iso2,결합기/지가류적Cas-L、Cbl、CrkL CENTD1( Centaurin deha1) ZO2,전록조절인자GFR-1급린산지매SHIP-2,저사린산화적단백견우Ph염색체양성ALL,기본불표체우기타ALL;③Hck、Lyn화Fgr선택성지여AML상관(M3제외).결론 ALL화AML존재상대특이적린산화다태,위급성백혈병적진단급치료제공료잠재적분자파위.
Objective To screen phosphopeptide specific for acute leukemia.Methods Mononuclear cells from bone marrow were collected from 16 newly diagnosed acute lymphoblastic leukemia (ALL) and 20 acute myeloid leukemia (AML) patients.Peptides were extracted and purified,analyzed by immunoprecipitation and liquid chromatography coupled with tandem mass spectrometry (LC-MS).Results ①Non-receptor tyrosine kinase family members Fyn,Yes,Src widely expressed in acute leukemia; ② Some phosphopeptides,including non-receptor tyrosine kinase family members Abl/iso1 and Abl,non-receptor Ser/Thr protein kinase family members Bcr,JNK2,JNK2 iso2,Adaptor/scaffold members Cas-L,Cbl, CrkL CENTD1 ( Centaurin deltal ) ZO2,transcriptor GFR-1 and phosphatase SHIP-2 were detected in Ph positive ALL,but not in other kinds of ALL.③)Hck,Lyn and Fgr selectively expressed in AML ( except AML-M3 ).Conclusion Some phosphopeptides were specific for ALL and AML,and may be useful for diagnosis and therapy of acute leukemia.