CAJ | 학술논문

目的研究缺血后处理是否可以减轻兔脊髓缺血-再灌注的损伤.方法雄性新西兰大白兔30只,随机分为五组,每组6只.假手术组(N1组)仅行单纯手术操作但不阻闭腹主动脉;对照组(N2组)行单纯缺血-再灌注;缺血后处理15s/30s/60s(PA/PB/PC组)分别于阻闭腹主动脉15rmin后,再灌注15s/30s/60s,缺血15s/30s/60s,反复3次.再灌注48 h时对所有动物的后肢运动功能进行评分并行脊髓前角正常神经元计数.结果PB组再灌注48 h后肢运动功能评分[3.5(2～4)分],明显高于N2组[2(1～3)分](P<0.05),其他各组与N2组相比差异无显著意义.脊髓前角正常神经元计数PB组为36.7±7.0,明显多于N2组25.7±4.3(P<0.01),而PA组18.2±2.2和PC组8.0±4.1则明显少于N2组(P<0.05).结论缺血后处理对兔脊髓缺血-再灌注损伤的作用取决于后处理时间,缺血后处理30s/30s对脊髓缺血-再灌注损伤具有保护作用,而缺血后处理15s/15s和60s/60s会加重脊髓损伤.
목적연구결혈후처리시부가이감경토척수결혈-재관주적손상.방법웅성신서란대백토30지,수궤분위오조,매조6지.가수술조(N1조)부행단순수술조작단불조폐복주동맥;대조조(N2조)행단순결혈-재관주;결혈후처리15s/30s/60s(PA/PB/PC조)분별우조폐복주동맥15rmin후,재관주15s/30s/60s,결혈15s/30s/60s,반복3차.재관주48 h시대소유동물적후지운동공능진행평분병행척수전각정상신경원계수.결과PB조재관주48 h후지운동공능평분[3.5(2～4)분],명현고우N2조[2(1～3)분](P<0.05),기타각조여N2조상비차이무현저의의.척수전각정상신경원계수PB조위36.7±7.0,명현다우N2조25.7±4.3(P<0.01),이PA조18.2±2.2화PC조8.0±4.1칙명현소우N2조(P<0.05).결론결혈후처리대토척수결혈-재관주손상적작용취결우후처리시간,결혈후처리30s/30s대척수결혈-재관주손상구유보호작용,이결혈후처리15s/15s화60s/60s회가중척수손상.