青岛大学医学院学报
青島大學醫學院學報
청도대학의학원학보
ACTA ACADEMIAE MEDICINAE QINGDAO UNIVERSITATIS
2007年
1期
31-33
,共3页
脊髓背角%蛋白激酶C%坐骨神经%免疫组织化学%大鼠
脊髓揹角%蛋白激酶C%坐骨神經%免疫組織化學%大鼠
척수배각%단백격매C%좌골신경%면역조직화학%대서
spinal dorsal horn%protein kinase C%sciatic nerve%immunohistochemistry%rat
目的 探讨大鼠坐骨神经切断模型脊髓背角内PKCγ的变化及意义.方法 建立大鼠坐骨神经切断模型,在坐骨神经切断后2、5、10、15、20、30、40、60 d,取脊髓背角,应用免疫组织化学方法进行染色,图像分析软件测量并比较脊髓背角手术侧和对照侧的免疫强度.结果 从第2天开始,手术侧脊髓背角L4～L5节段PKCγ免疫阳性反应较对照侧明显增强,差异有显著性(t=3.12,P＜0.05);在第15天时免疫阳性反应最强,是对照组的1.89倍(t=4.85,P＜0.01),然后逐渐减弱,2个月后恢复到正常水平(t=0.91,P＞0.05).结论 PKCγ在由神经损伤引起的脊髓背角神经元兴奋性改变中发挥重要作用.
目的 探討大鼠坐骨神經切斷模型脊髓揹角內PKCγ的變化及意義.方法 建立大鼠坐骨神經切斷模型,在坐骨神經切斷後2、5、10、15、20、30、40、60 d,取脊髓揹角,應用免疫組織化學方法進行染色,圖像分析軟件測量併比較脊髓揹角手術側和對照側的免疫彊度.結果 從第2天開始,手術側脊髓揹角L4～L5節段PKCγ免疫暘性反應較對照側明顯增彊,差異有顯著性(t=3.12,P＜0.05);在第15天時免疫暘性反應最彊,是對照組的1.89倍(t=4.85,P＜0.01),然後逐漸減弱,2箇月後恢複到正常水平(t=0.91,P＞0.05).結論 PKCγ在由神經損傷引起的脊髓揹角神經元興奮性改變中髮揮重要作用.
목적 탐토대서좌골신경절단모형척수배각내PKCγ적변화급의의.방법 건립대서좌골신경절단모형,재좌골신경절단후2、5、10、15、20、30、40、60 d,취척수배각,응용면역조직화학방법진행염색,도상분석연건측량병비교척수배각수술측화대조측적면역강도.결과 종제2천개시,수술측척수배각L4～L5절단PKCγ면역양성반응교대조측명현증강,차이유현저성(t=3.12,P＜0.05);재제15천시면역양성반응최강,시대조조적1.89배(t=4.85,P＜0.01),연후축점감약,2개월후회복도정상수평(t=0.91,P＞0.05).결론 PKCγ재유신경손상인기적척수배각신경원흥강성개변중발휘중요작용.
Objective To investigate the pain-related changes and possible function of protein kinase Cγ (PKCγ) in the rat spinal dorsal horn following sciatic nerve axotomy. Methods The density of PKCγ immunoreactivity in the dorsal horn following sciatic nerve transection was valued in different time after surgery, 2,5,10,15,20,30,40, and 60 days later respectively. The value (light absorbance value) was analyzed by contrasting the contralateral side and ipsilateral side with a computer-assisted imaging analysis system. Results Axotomy of sciatic nerve significantly increased PKCγ immunoreactivity in the contralateral superficial dorsal horn of the L4 and L5 segments two to 40 days after the transection. Two days later, immunoreactivity in contrala-teral side had changed significantly (t=3.12,P＜0.05). This value reached 1.89 times highest in contralateral side 15 days later (t=4.85,P＜0.01), while after a period of two months it returned to the normal level (t=0.91,P＞0.05). Conclusion PKCγ plays an important role in the changes of excitability of dorsal horn neurons caused by nerve injury.
