CAJ | 학술논문

获得高质量蛋白质晶体一直是X射线衍射解析蛋白质结构的瓶颈问题,因此研究提高蛋白质晶体质量的方法具有非常重要的意义.近年来人们从影响蛋白质结晶过程的各个因素出发,发展了一系列提高蛋白质晶体质量的方法,如在亚稳区生长蛋白质晶体、利用特殊环境影响蛋白质晶体生长的动力学过程、利用去垢剂提高蛋白质晶体质量和分子工程改造蛋白质分子提高晶体质量等.分析和评述了这些方法影响蛋白质结晶过程的机理.
획득고질량단백질정체일직시X사선연사해석단백질결구적병경문제,인차연구제고단백질정체질량적방법구유비상중요적의의.근년래인문종영향단백질결정과정적각개인소출발,발전료일계렬제고단백질정체질량적방법,여재아은구생장단백질정체、이용특수배경영향단백질정체생장적동역학과정、이용거구제제고단백질정체질량화분자공정개조단백질분자제고정체질량등.분석화평술료저사방법영향단백질결정과정적궤리.
Obtaining high quality crystal of protein has always been the bottleneck of structure determination by X-ray crystallography. In recent years, crystallographers have developed a series of method to improve the quality of protein crystals, such as crystal growth in metastable zone, influencing the kinetics process of protein crystal in special environment, obtaining high quality crystals by adding detergent and reductive methylation of proteins. In this paper,the principle of improving the protein crystal quality is discussed and reviewed.