哈尔滨医科大学学报
哈爾濱醫科大學學報
합이빈의과대학학보
JOURNAL OF HARBIN MEDICAL UNIVERSITY
2010年
1期
22-25
,共4页
新生小鼠%肌源性干细胞%表型%细胞增殖
新生小鼠%肌源性榦細胞%錶型%細胞增殖
신생소서%기원성간세포%표형%세포증식
newborn mice%muscle derived stem cells%phenotype identification%cell proliferation
目的 通过体外新生小鼠肌源性干细胞(MDSCs)的培养,观察鼠肌源干细胞的生物学特性及表型.方法 采用胶原酶Ⅺ和胰蛋白酶分步消化肌肉, 后采用差速贴壁分离技术纯化获得MDSCs.用倒置显微镜观察细胞形态,测定生长曲线,分析MDSCs的增殖能力,采用Sca-1、CD34 和Desmin免疫细胞化学染色对细胞进行鉴定,初步确定细胞表型.结果 经过两步消化和差速贴壁后,成功培养出高纯度的肌源性干细胞,该细胞呈Sca-1、CD34和Desmin阳性.结论 可通过体外原代培养获得高纯度的肌源性干细胞,细胞具有良好的增殖能力,是适用于组织工程和基因治疗研究的一种新型种子细胞.
目的 通過體外新生小鼠肌源性榦細胞(MDSCs)的培養,觀察鼠肌源榦細胞的生物學特性及錶型.方法 採用膠原酶Ⅺ和胰蛋白酶分步消化肌肉, 後採用差速貼壁分離技術純化穫得MDSCs.用倒置顯微鏡觀察細胞形態,測定生長麯線,分析MDSCs的增殖能力,採用Sca-1、CD34 和Desmin免疫細胞化學染色對細胞進行鑒定,初步確定細胞錶型.結果 經過兩步消化和差速貼壁後,成功培養齣高純度的肌源性榦細胞,該細胞呈Sca-1、CD34和Desmin暘性.結論 可通過體外原代培養穫得高純度的肌源性榦細胞,細胞具有良好的增殖能力,是適用于組織工程和基因治療研究的一種新型種子細胞.
목적 통과체외신생소서기원성간세포(MDSCs)적배양,관찰서기원간세포적생물학특성급표형.방법 채용효원매Ⅺ화이단백매분보소화기육, 후채용차속첩벽분리기술순화획득MDSCs.용도치현미경관찰세포형태,측정생장곡선,분석MDSCs적증식능력,채용Sca-1、CD34 화Desmin면역세포화학염색대세포진행감정,초보학정세포표형.결과 경과량보소화화차속첩벽후,성공배양출고순도적기원성간세포,해세포정Sca-1、CD34화Desmin양성.결론 가통과체외원대배양획득고순도적기원성간세포,세포구유량호적증식능력,시괄용우조직공정화기인치료연구적일충신형충자세포.
Objective To explore the feasibility of isolation and characteristics of the muscle-derived stem cells (MDSCs) of newborn mice. Methods To get purified MDSCs, newborn mice muscle were digested to single cells by collagenase and trypsin digestion, then were separated through differential adhesion. MDSCs were observed morphology under inverted microscope, reproductive activity was analyzed by determining growth curves and cells were identified by immunostaining with anti-Sca-1, CD34 and Desmin antibodies. Results Highly purified MDSCs were harvested and showed Sca-1(+)、CD34(+) and Desmin(+). Conclusion MDSCs can be isolated and cultured in vitro. MDSCs possess good proliferative ability and is adaptable for tissue engineering and gene therapy.
