CAJ | 학술논문

为研究鸡卡氏杆菌引起输卵管囊肿的病理学变化,对9例自然感染病例进行症状观察,病理剖检,并采集气管、肺脏、输卵管和卵巢组织,从组织中提取基因组DNA,采用卡氏杆菌属特异性引物,用PCR方法成功扩增出卡氏杆菌目的基因;经PCR鉴定的卡氏杆菌阳性病料经甲醛固定,梯度乙醇脱水,石蜡切片,HE染色,光镜观察.结果表明:呼吸道的病变主要在黏膜层,黏膜上皮细胞脱落,固有层内毛细血管充血,炎性细胞浸润;输卵管主要表现在膨大和子宫的黏膜层有大量炎性细胞浸润,且黏膜上皮分泌细胞的细胞核破裂.输卵管的病变可作为该病病理诊断的重要指征之一,为阐明该病的输卵管特征性病理变化及进一步探讨其发生机制奠定了基础.
위연구계잡씨간균인기수란관낭종적병이학변화,대9례자연감염병례진행증상관찰,병리부검,병채집기관、폐장、수란관화란소조직,종조직중제취기인조DNA,채용잡씨간균속특이성인물,용PCR방법성공확증출잡씨간균목적기인;경PCR감정적잡씨간균양성병료경갑철고정,제도을순탈수,석사절편,HE염색,광경관찰.결과표명:호흡도적병변주요재점막층,점막상피세포탈락,고유층내모세혈관충혈,염성세포침윤;수란관주요표현재팽대화자궁적점막층유대량염성세포침윤,차점막상피분비세포적세포핵파렬.수란관적병변가작위해병병리진단적중요지정지일,위천명해병적수란관특정성병리변화급진일보탐토기발생궤제전정료기출.
In order to observe the histopathological changes of the layer oviduct cysts infected with Gallibacterium anatis,target genes were amplified by PCR from genome DNA extracted from lung, trachea, oviduct and ovary of nine birds infected naturally, using genus-specificity primes of Gallibacterium anatis, and the Gallibacterium anatis PCR positive samples were fixed in the formalin and dehydrated with gradient alcohol. The paraffin sections were made and stained with haematoxyylin and eosin, then were observed by light microscope. The results showed that Gallibacterium anatis mainly induced exfoliation of the mucosa epithelium cells, hyperaemia of cortex capillary and infiltration of inflammation cells in up-respiratory tract, as well as inflammation cells in mucosa of magnum and uterus in oviduct and nucleus of secretory cells broken into small pieces. This pathological changes could be one of the important diagnosis points of the disease, which laid the foundation for elucidating the characteristic pathological changes of oviduct and the development mechanism of the disease.