中国组织化学与细胞化学杂志
中國組織化學與細胞化學雜誌
중국조직화학여세포화학잡지
CHINESE JOURNAL OF HISTOCHEMISY AND CYTOCHEMISY
2010年
1期
53-57
,共5页
李桂玲%归绥琪%夏晴%陈松华%葛锡锐%林理根%叶阳
李桂玲%歸綏琪%夏晴%陳鬆華%葛錫銳%林理根%葉暘
리계령%귀수기%하청%진송화%갈석예%림리근%협양
掌叶半夏提取物%子宫颈癌%CaSK细胞株%Hela细胞株%细胞外信号调节蛋白激酶
掌葉半夏提取物%子宮頸癌%CaSK細胞株%Hela細胞株%細胞外信號調節蛋白激酶
장협반하제취물%자궁경암%CaSK세포주%Hela세포주%세포외신호조절단백격매
Pinellia extract%Cervical cancer%CaSki cell line%HeLa cell line%Exfernal Signal-regulated xinase p-ERK
目的 既往的临床研究证实中药掌叶半夏可有效治疗宫颈癌.在此基础上,课题组观察了中药掌叶半夏有效提取物(Pinellia Extract,PE)对宫颈癌细胞株CaSki和HeLa细胞增殖的作用,先前采用MTT法检测细胞活性以及倒置相差显微镜观察细胞形态的变化,初步显示了PE对CaSki和HeLa细胞的增殖有抑制作用;本文将对PE的增殖抑制作用进行进一步的研究.方法 采用扫描电镜和透射电镜观察PE作用后CaSki和HeLa细胞表面及内部超微结构的变化,In-cell Western blot检测不同浓度PE作用15min后CaSki和HeLa细胞磷酸化ERK的表达.结果 0.15mg/ml PE作用24h后CaSki和HeLa细胞表面结构及内部结构均发生了显著的变化,提示细胞增殖受到抑制;不同浓度PE作用15min后CaSki和HeLa细胞磷酸化ERK的表达随PE浓度的增加逐渐下降.结论 PE对CaSki和HeLa宫颈癌细胞株的增殖有明显抑制作用,表现在细胞表面及内部超微结构的变化;增殖抑制作用之一是阻断ERK的磷酸化.实验结果为进一步研究PE的抗宫颈癌作用机制以及新药的研制开发奠定了一定的理论依据和实验基础.
目的 既往的臨床研究證實中藥掌葉半夏可有效治療宮頸癌.在此基礎上,課題組觀察瞭中藥掌葉半夏有效提取物(Pinellia Extract,PE)對宮頸癌細胞株CaSki和HeLa細胞增殖的作用,先前採用MTT法檢測細胞活性以及倒置相差顯微鏡觀察細胞形態的變化,初步顯示瞭PE對CaSki和HeLa細胞的增殖有抑製作用;本文將對PE的增殖抑製作用進行進一步的研究.方法 採用掃描電鏡和透射電鏡觀察PE作用後CaSki和HeLa細胞錶麵及內部超微結構的變化,In-cell Western blot檢測不同濃度PE作用15min後CaSki和HeLa細胞燐痠化ERK的錶達.結果 0.15mg/ml PE作用24h後CaSki和HeLa細胞錶麵結構及內部結構均髮生瞭顯著的變化,提示細胞增殖受到抑製;不同濃度PE作用15min後CaSki和HeLa細胞燐痠化ERK的錶達隨PE濃度的增加逐漸下降.結論 PE對CaSki和HeLa宮頸癌細胞株的增殖有明顯抑製作用,錶現在細胞錶麵及內部超微結構的變化;增殖抑製作用之一是阻斷ERK的燐痠化.實驗結果為進一步研究PE的抗宮頸癌作用機製以及新藥的研製開髮奠定瞭一定的理論依據和實驗基礎.
목적 기왕적림상연구증실중약장협반하가유효치료궁경암.재차기출상,과제조관찰료중약장협반하유효제취물(Pinellia Extract,PE)대궁경암세포주CaSki화HeLa세포증식적작용,선전채용MTT법검측세포활성이급도치상차현미경관찰세포형태적변화,초보현시료PE대CaSki화HeLa세포적증식유억제작용;본문장대PE적증식억제작용진행진일보적연구.방법 채용소묘전경화투사전경관찰PE작용후CaSki화HeLa세포표면급내부초미결구적변화,In-cell Western blot검측불동농도PE작용15min후CaSki화HeLa세포린산화ERK적표체.결과 0.15mg/ml PE작용24h후CaSki화HeLa세포표면결구급내부결구균발생료현저적변화,제시세포증식수도억제;불동농도PE작용15min후CaSki화HeLa세포린산화ERK적표체수PE농도적증가축점하강.결론 PE대CaSki화HeLa궁경암세포주적증식유명현억제작용,표현재세포표면급내부초미결구적변화;증식억제작용지일시조단ERK적린산화.실험결과위진일보연구PE적항궁경암작용궤제이급신약적연제개발전정료일정적이론의거화실험기출.
Objective To investigate the effects of Pinellia extract (PE) on cervical cancer cell lines CaSki and HeLa cultured in vitro, based on the previous research of Pinellia's effective treatment of cervical cancer. Methods Changes of ultrastructure were observed by electron microscopy. In-cell Western blot was used to estimate the relative protein amounts of phosphorylated ERK of CaSki and HeLa cells treated with different concentrations of PE for 15 minutes.Results PE inhibited the proliferation of CaSki and HeLa cells in a time- and dose-dependent manner. Electron microscopy revealed that the morphology and ultrastructure of CaSki and HeLa cells changed a lot. PE inhibited the phosphorylation of ERK in a dose-dependent manner.Conclusion PE can suppress proliferation of cervical cancer cells and induce changes in morphology and ultrastructure of CaSki and HeLa. Blocking of the MAPK/ERK signal transduction pathway by PE is involved in the mechanisms. This result will provide a certain experimental basis for the research of PE's antitumor effects.
