CAJ | 학술논문

目的观察中药掌叶半夏有效提取物(Pinellia extract,PE)对HeLa细胞的作用,并对可能的机制进行初步研究,为其临床用于防治女性宫颈病变提供科学依据.方法用倒置相差显微镜观察细胞生长并摄影,MTT法检测细胞活性,流式细胞术检测细胞凋亡,免疫细胞化学法检测增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)蛋白表达,免疫荧光实验检测B细胞淋巴瘤/白血病-2(Bcl-2)蛋白表达.结果 PE对HeLa细胞的生长表现出明显的抑制作用,流式细胞术检测结果显示PE作用后HeLa细胞出现凋亡,且随着作用时间延长,凋亡细胞的比例明显增加;免疫细胞化学实验和免疫荧光实验结果表明:PE作用24 h后HeLa细胞PCNA和Bcl-2蛋白表达均明显减少.结论 PE对HeLa细胞有明显的抑制生长和促进凋亡的作用,其促凋亡机制是通过降低PCNA和Bcl-2蛋白表达来实现的,这为进一步研究PE的作用机制以及抗癌新药的研制开发奠定了初步的实验基础.
목적 관찰중약장협반하유효제취물(Pinellia extract,PE)대HeLa세포적작용,병대가능적궤제진행초보연구,위기림상용우방치녀성궁경병변제공과학의거.방법 용도치상차현미경관찰세포생장병섭영,MTT법검측세포활성,류식세포술검측세포조망,면역세포화학법검측증식세포핵항원(proliferating cell nuclear antigen,PCNA)단백표체,면역형광실험검측B세포림파류/백혈병-2(Bcl-2)단백표체.결과 PE대HeLa세포적생장표현출명현적억제작용,류식세포술검측결과현시PE작용후HeLa세포출현조망,차수착작용시간연장,조망세포적비례명현증가;면역세포화학실험화면역형광실험결과표명:PE작용24 h후HeLa세포PCNA화Bcl-2단백표체균명현감소.결론 PE대HeLa세포유명현적억제생장화촉진조망적작용,기촉조망궤제시통과강저PCNA화Bcl-2단백표체래실현적,저위진일보연구PE적작용궤제이급항암신약적연제개발전정료초보적실험기출.
Objective To investigate the effect of Pinellia extract (PE) on HeLa cell line and to study its associated mechanisms,in order to provide theoretical foundations for its applying in clinical prevention and treatment of cervical disease.Methods HeLa cell line was incubated in media containing different concentrations of PE.Growth of cells was observed and photo-generated with inverted phase microscope;cell activity was detected by MTT assay;cell apoptosis detected by flow cytometry,protein expression of proliferating cell nuclear antigen proliferating cell nuclear antigen (PCNA) was detected by immuno-cytochemistry,and protein expression of Bcl-2 was determined by immunofluorescence.Results PE showed obvious inhibition on the proliferation of HeLa cells,cell apoptosis appeared after PE treatment in a time and dose dependent manner;PCNA and Bcl-2 protein expressions reduced significantly after being effected by PE for 24 h.Conclusions PE can obviously inhibit the growth and induce the apoptosis of HeLa cells,and its mechanism is possibly realized through down-regulating the expressions of PCNA and Bcl-2.The study set a primary experimental base for further studying the action mechanism of PE and developing new anti-tumor agents.