CAJ | 학술논문

建立测定水产品中残留的甲氧苄啶的高效液相色谱法.样品经三氯甲烷和酸性甲醇溶液提取,提取液调至碱性后用二氯甲烷萃取,萃取液经MCX柱净化,洗脱液浓缩至近干后用流动相溶解,过微孔滤膜,采用高效液相色谱紫外检测,外标法定量.结果表明,甲氧_苄啶在0.05～5.0μg/mL质量浓度范围内,色谱峰面积与甲氧苄啶质量浓度之间线性关系良好(相关系数为0.999967),在空白样品的20、40、200μg/kg 3个加标晕条件下,样品平均回收率均大于80%,相对标准偏差小于7%,方法的检测限为20μg/kg.该方法简便快速,基体干扰小,精密度、准确性均能满足水产品中甲氧苄啶的残留分析.
건립측정수산품중잔류적갑양변정적고효액상색보법.양품경삼록갑완화산성갑순용액제취,제취액조지감성후용이록갑완췌취,췌취액경MCX주정화,세탈액농축지근간후용류동상용해,과미공려막,채용고효액상색보자외검측,외표법정량.결과표명,갑양_변정재0.05～5.0μg/mL질량농도범위내,색보봉면적여갑양변정질량농도지간선성관계량호(상관계수위0.999967),재공백양품적20、40、200μg/kg 3개가표훈조건하,양품평균회수솔균대우80%,상대표준편차소우7%,방법적검측한위20μg/kg.해방법간편쾌속,기체간우소,정밀도、준학성균능만족수산품중갑양변정적잔류분석.
A reversed-phase high performance liquid chromatographic (RP-HPLC) method was developed for the determination of trimethoprim residue in aquatic products.Samples were extracted with a mixture of chloroform,sulfuric acid and methanol (15:14:6,V/V).The resulting extraction solution was alkalized,reextracted with dichloromethane and cleaned up on MCX column.The eluate was pooled,concentrated nearly to dryness,rediluted with the HPLC mobile phase and filtrated through a 0.45 μm pore size membrane.Trimethoprim was detected with an ultraviolet (UV) detector and quantified by external standard method.Good linearity between chromatographic peak area and trimethoprim concentration ranging from 0.05 to 5.0 μg/mL was found (R~2 = 0.999967).Average recoveries of trimethoprim in different blank aquatic products spiked at the levels of 20,40,200 μg/kg were all more than 80%,with relative standard deviations of less than 7%.The method exhibited a detection limit of 20 μg/kg.Due to the advantages of simplicity,rapidity,little matrix interference and high accuracy and precision,this method is most suitable for the determination of trimethoprim residue in aquatic products.