CAJ | 학술논문

采用Sephadex-G50凝胶柱层析分离具有强抗氧化活性的小分子芸豆肽,以VC为对照,研究3种肽分离组分的还原能力及对羟自由基、超氧阴离子自由基和DPPH自由基的清除能力.结果表明:随着相对分子质量的减小和肽质量浓度的增加,其还原能力及对羟自由基、超氧阴离子自由基和DPPH自由基的清除能力逐渐增大;小分子芸豆肽(C组分)的还原能力低于VC;对羟自由基、超氧阴离子自由基和DPPH自由基的半清除质量浓度分别为2.301、2.553、1.386mg/mL.该小分子肽组分的相对分子质量主要集中在100～1000,该组分疏水性氨基酸含量为36.78%,其疏水值达到364.73 kcal/mol.说明小分子肽组分的抗氧化活性与其氨基酸种类和组成、相对分子质量分布密切相关.
채용Sephadex-G50응효주층석분리구유강항양화활성적소분자예두태,이VC위대조,연구3충태분리조분적환원능력급대간자유기、초양음리자자유기화DPPH자유기적청제능력.결과표명:수착상대분자질량적감소화태질량농도적증가,기환원능력급대간자유기、초양음리자자유기화DPPH자유기적청제능력축점증대;소분자예두태(C조분)적환원능력저우VC;대간자유기、초양음리자자유기화DPPH자유기적반청제질량농도분별위2.301、2.553、1.386mg/mL.해소분자태조분적상대분자질량주요집중재100～1000,해조분소수성안기산함량위36.78%,기소수치체도364.73 kcal/mol.설명소분자태조분적항양화활성여기안기산충류화조성、상대분자질량분포밀절상관.
Kidney bean peptides (KBP) were fractionated by gel filtration on SephadexG-50.The antioxidant and free radical-scavenging activities of three KBP fractions (Fra-A, Fra-B, Fra-C) were investigated using reducing power and 1,1-diphenyl- 2-pycrylhydrazyl (DPPH)/superoxide/hydroxyl radical-scavenging assay. The reducing power activity of Fra-C (RP50 2.263 mg/mL) was lower than that of ascorbic acid (RP50 0.828 mg/mL). Fra-C showed the strongest scavenging activity against DPPH,superoxideanion and hydroxyl radicals. The radical-scavenging effect was in a dose-dependent manner and the EC50 values for DPPH, superoxide and hydroxyl radicals were found to be 2.301, 2.553 mg/mL and 1.386 mg/mL, respectively. The amino acid analysis showed that Fra-C had a hydrophobic amino acid content of 36.78% and a hydrophobicity of 364.73 kcal/mol.The molecular weight distribution of Fra-C was found to mainly vary from 100 to 1000. The antioxidant activity of Fra-C is dearly related to the amino acid composition, the content of THAA and the molecular mass.