CAJ | 학술논문

目的探讨细胞周期蛋白(Cyclin)D1在三氧化二砷(As2O3)抑制人胆囊癌细胞体外生长中的作用.方法不同浓度的As2O3作用于胆囊癌细胞GBC-SD,MTT法检测细胞生长情况,流式细胞仪检测细胞周期分布.Western印迹和RT-PCR检测Cyclin D1、D2、D3和CDK4、K6的蛋白和mRNA表达.构建Cyclin D1的表达质粒并转染GBC细胞,观察Cyclin D1过表达时细胞周期的变化.结果 As2O3能抑制胆囊癌细胞生长,作用呈时间、剂量一效应关系;流式细胞仪检测发现As2O3可将增殖过程中的胆囊癌细胞阻滞于G1期;Western印迹及RT-PCR检测显示Cyclin D1表达下降;过表达Cyelin D1逆转了As2O3对细胞周期的改变.结论 Cyclin D1在As2O3抑制人胆囊癌细胞的生长中起重要作用.
목적 탐토세포주기단백(Cyclin)D1재삼양화이신(As2O3)억제인담낭암세포체외생장중적작용.방법 불동농도적As2O3작용우담낭암세포GBC-SD,MTT법검측세포생장정황,류식세포의검측세포주기분포.Western인적화RT-PCR검측Cyclin D1、D2、D3화CDK4、K6적단백화mRNA표체.구건Cyclin D1적표체질립병전염GBC세포,관찰Cyclin D1과표체시세포주기적변화.결과 As2O3능억제담낭암세포생장,작용정시간、제량일효응관계;류식세포의검측발현As2O3가장증식과정중적담낭암세포조체우G1기;Western인적급RT-PCR검측현시Cyclin D1표체하강;과표체Cyelin D1역전료As2O3대세포주기적개변.결론 Cyclin D1재As2O3억제인담낭암세포적생장중기중요작용.
Objective To investigate the role of the cyclin D1 in inhibition of human gallbladder carcinoma(hGBC) cells by arsenic trioxide(As2O3). Methods After the hGBC cells were cultured with different concentrations of As2O3, the proliferative activity of cells was detected by MTT meth-ods, the cell cycle status determined by flow cytometry as well as the protein and mRNA expression of Cyclin D1,D2,D3,CDK4 and CDK6 analyzed by Western blot and RT-PCR. hGBC cells were transi-ently transfeeted with overexpression of Cyclin D1 derived from the constructed plasmid. Meanwhile, the As2O3-induced alteration of cell cycle was detected. Results The As2O3could inhibit the growth of cells in a time-and dose-dependent manner, make ceils arrested in G, and downregulate the expres-sion of Cyclin D1. Moreover, Cyclin D1 overexpression played the negative role in As2O3-induced al-teration of cell cycle. Conclusion Cyelin D1 plays an important role in inhibition of hGBC cells by As2O3.